| General Information |
| MoonProt ID | 219 |
| First appeared in release | 1.0 |
| Name(s) | Thymidylate synthase
5,10-methylenetetrahydrofolate:dUMP C-methyltransferase
TS
TSase
Gene Name:TYMS |
| UniProt ID | P04818 (TYSY_HUMAN) Reviewed |
| GO terms | GO:0000082 G1/S transition of mitotic cell cycle
GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:0000278 mitotic cell cycle
GO:0005976 polysaccharide metabolic process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006206 pyrimidine nucleobase metabolic process
GO:0006231 dTMP biosynthetic process
GO:0006235 dTTP biosynthetic process
GO:0006260 DNA replication
GO:0006281 DNA repair
GO:0007568 aging
GO:0007623 circadian rhythm
GO:0009157 deoxyribonucleoside monophosphate biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0009636 response to toxic substance
GO:0014070 response to organic cyclic compound
GO:0019088 immortalization of host cell by virus
GO:0019860 uracil metabolic process
GO:0031100 organ regeneration
GO:0032259 methylation
GO:0032570 response to progesterone
GO:0033189 response to vitamin A
GO:0034097 response to cytokine
GO:0042493 response to drug
GO:0044281 small molecule metabolic process
GO:0045471 response to ethanol
GO:0046078 dUMP metabolic process
GO:0046134 pyrimidine nucleoside biosynthetic process
GO:0046653 tetrahydrofolate metabolic process
GO:0046683 response to organophosphorus
GO:0048015 phosphatidylinositol-mediated signaling
GO:0048589 developmental growth
GO:0051216 cartilage development
GO:0051384 response to glucocorticoid
GO:0051593 response to folic acid
GO:0055086 nucleobase-containing small molecule metabolic process
GO:0060574 intestinal epithelial cell maturation
GO:0000166 nucleotide binding
GO:0003729 mRNA binding
GO:0004799 thymidylate synthase activity
GO:0005542 folic acid binding
GO:0008144 drug binding
GO:0008168 methyltransferase activity
GO:0016740 transferase activity
GO:0042803 protein homodimerization activity
GO:0048037 cofactor binding
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005759 mitochondrial matrix
GO:0005829 cytosol
GO:0016020 membrane
|
| Organisms for which functions have been demonstrated | Homo sapiens (human, a mammal, OMIM number 188350 ) |
| Sequence length | 313 |
| FASTA sequence | >sp|P04818|TYSY_HUMAN Thymidylate synthase OS=Homo sapiens GN=TYMS PE=1 SV=3
MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV
|
| Structure Information |
| PDB ID | 2RD8,
2RDA,
3H9K,
3HB8,
3OB7,
4GYH,
4H1I,
3EF9,
3GH0,
1JU6,
1JUJ,
3EDW,
3EJL,
3GG5,
3GH24E28,
3N5E,
3EAW,
4G2O,
4G6W,
3EHI,
4FGT,
4GD7,
3N5E,
3EBU,
1HW4,
1HW3,
1YPV,
2ONB,
3N5G,
3EGY,
3ED7,
1HVY,
1HZW,
1I00 |
| Quaternary structure | |
| SCOP | Thymidylate synthase/dCMP hydroxymethylase |
| CATH | 3.30.572.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | 15.65%, 1-27, 107-128, |
| Predicted Disorder Regions | 1 to 26 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | thymidylate synthase, enzyme
de novo synthesis of thymidylate, a DNA precursor
5,10-methylenetetrahydrofolate + dUMP <=> dihydrofolate + dTMP
Pyrimidine metabolism, dTTP biosynthesis |
| References for function | |
| E.C. number | 2.1.1.45 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | More complete list of PDB IDs: 2RD8, 2RDA, 3H9K, 3HB8, 3OB7, 4GYH, 4H1I, 3EF9, 3GH0, 1JU6, 1JUJ, 3EDW, 3EJL, 3GG5, 3GH2, 4E28, 3N5E, 3EAW, 4G2O, 4G6W, 3EHI, 4FGT, 4GD7, 3N5E, 3EBU, 1HW4, 1HW3, 1YPV, 2ONB, 3N5G, 3EGY, 3ED7, 1HVY, 1HZW, 1I00 |
| Function 2 |
| Function description | mRNA translation inhibition
regulation of human TS mRNA translation
does not bind to unrelated mRNAs
|
| References for function | Chu E, Koeller DM, Casey JL, Drake JC, Chabner BA, Elwood PC, Zinn S, Allegra CJ. Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase. Proc Natl Acad Sci U S A. 1991 Oct 15. PMID: 1924359 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |