Protein Information

General Information
MoonProt ID138
First appeared in release1.0
Name(s)birA biotin synthetase Bifunctional protein BirA Bifunctional ligase/repressor BirA Biotin operon repressor Biotin-[acetyl-CoA-carboxylase] ligase Biotin-protein ligase Biotin-[acetyl-CoA carboxylase] synthetase Gene Name: birA
UniProt IDP06709 (BIRA_ECOLI) Reviewed
GO termsGO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006464 cellular protein modification process GO:0009102 biotin biosynthetic process GO:0009305 protein biotinylation GO:0000166 nucleotide binding GO:0003677 DNA binding GO:0003824 catalytic activity GO:0004077 biotin-[acetyl-CoA-carboxylase] ligase activity GO:0005524 ATP binding GO:0016874 ligase activity
Organisms for which functions have been demonstratedEscherichia coli
Sequence length321
FASTA sequence>sp|P06709|BIRA_ECOLI Bifunctional ligase/repressor BirA OS=Escherichia coli (strain K12) GN=birA PE=1 SV=1 MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
Structure Information
PDB ID
Quaternary structure
Function 1
Function descriptionbirA biotin synthetase, enzyme biotin--[acetyl-CoA-carboxylase] ligase ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].
References for function
E.C. number6.3.4.15
Location of functional site(s)
Cellular location of function
Comments
Function 2
Function descriptionbio operon repressor repressor activity depends on cellular concentration of biotin
References for functionBarker DF, Campbell AM. Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol. 1981 Mar 15. PMID: 6456358
E.C. numberN/A
Location of functional site(s)
Cellular location of functionbound to DNA
Comments