| General Information |
| MoonProt ID | 138 |
| First appeared in release | 1.0 |
| Name(s) | birA biotin synthetase
Bifunctional protein BirA
Bifunctional ligase/repressor BirA
Biotin operon repressor
Biotin-[acetyl-CoA-carboxylase] ligase
Biotin-protein ligase
Biotin-[acetyl-CoA carboxylase] synthetase
Gene Name: birA |
| UniProt ID | P06709 (BIRA_ECOLI) Reviewed |
| GO terms | GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006464 cellular protein modification process
GO:0009102 biotin biosynthetic process
GO:0009305 protein biotinylation
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004077 biotin-[acetyl-CoA-carboxylase] ligase activity GO:0005524 ATP binding
GO:0016874 ligase activity |
| Organisms for which functions have been demonstrated | Escherichia coli (Gram negative bacterium) |
| Sequence length | 321 |
| FASTA sequence | >sp|P06709|BIRA_ECOLI Bifunctional ligase/repressor BirA OS=Escherichia coli (strain K12) GN=birA PE=1 SV=1
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK |
| Structure Information |
| PDB ID | 1BIA_A; 100% Query Cover |
| Quaternary structure | |
| SCOP | NA |
| CATH | 1.10.10.10, 3.30.930.10, 2.30.30.100 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | 7.17%,212 to 234 |
| Predicted Disorder Regions | 1 to 5, 319-321 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | birA biotin synthetase, enzyme
biotin--[acetyl-CoA-carboxylase] ligase
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]. |
| References for function | |
| E.C. number | 6.3.4.15 |
| Location of functional site(s) | |
| Cellular location of function | |
| Comments | |
| Function 2 |
| Function description | bio operon repressor
repressor activity depends on cellular concentration of biotin |
| References for function | Barker DF, Campbell AM. Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol. 1981 Mar 15. PMID: 6456358 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | bound to DNA |
| Comments | |