General Information |
MoonProt ID | 138 |
First appeared in release | 1.0 |
Name(s) | birA biotin synthetase
Bifunctional protein BirA
Bifunctional ligase/repressor BirA
Biotin operon repressor
Biotin-[acetyl-CoA-carboxylase] ligase
Biotin-protein ligase
Biotin-[acetyl-CoA carboxylase] synthetase
Gene Name: birA |
UniProt ID | P06709 (BIRA_ECOLI) Reviewed |
GO terms | GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006464 cellular protein modification process
GO:0009102 biotin biosynthetic process
GO:0009305 protein biotinylation
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004077 biotin-[acetyl-CoA-carboxylase] ligase activity GO:0005524 ATP binding
GO:0016874 ligase activity |
Organisms for which functions have been demonstrated | Escherichia coli (Gram negative bacterium) |
Sequence length | 321 |
FASTA sequence | >sp|P06709|BIRA_ECOLI Bifunctional ligase/repressor BirA OS=Escherichia coli (strain K12) GN=birA PE=1 SV=1
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK |
Structure Information |
PDB ID | 1BIA_A; 100% Query Cover |
Quaternary structure | |
SCOP | NA |
CATH | 1.10.10.10, 3.30.930.10, 2.30.30.100 |
TM Helix Prediction | no TM helices |
DisProt Annotation | 7.17%,212 to 234 |
Predicted Disorder Regions | 1 to 5, 319-321 |
Connections to Disease |
OMIM ID | |
Function 1 |
Function description | birA biotin synthetase, enzyme
biotin--[acetyl-CoA-carboxylase] ligase
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]. |
References for function | |
E.C. number | 6.3.4.15 |
Location of functional site(s) | |
Cellular location of function | |
Comments | |
Function 2 |
Function description | bio operon repressor
repressor activity depends on cellular concentration of biotin |
References for function | Barker DF, Campbell AM. Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol. 1981 Mar 15. PMID: 6456358 |
E.C. number | N/A |
Location of functional site(s) | |
Cellular location of function | bound to DNA |
Comments | |