| General Information |
| MoonProt ID | 147 |
| First appeared in release | 1.0 |
| Name(s) | Transketolase 1
TktA
Gene Name: tktA |
| UniProt ID | P27302 (TKT1_ECOLI), Reviewed |
| GO terms | GO:0008152 metabolic process
GO:0009052 pentose-phosphate shunt, non-oxidative branch
GO:0003824 catalytic activity
GO:0004802 transketolase activity
GO:0016740 transferase activity
GO:0030145 manganese ion binding
GO:0030976 thiamine pyrophosphate binding
GO:0046872 metal ion binding |
| Organisms for which functions have been demonstrated | Escherichia coli (Gram negative bacterium) |
| Sequence length | 663 |
| FASTA sequence | >sp|P27302|TKT1_ECOLI Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL
|
| Structure Information |
| PDB ID | 2R5N |
| Quaternary structure | |
| SCOP | Thiamin diphosphate-binding fold (THDP-binding), TK C-terminal domain-like |
| CATH | 3.40.50.970, 3.40.50.920 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1 to 5, 254-258, 306-308 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | transketolase, enzyme
Pentose Phosphate Pathway
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate <=> D-ribose 5-phosphate + D-xylulose 5-phosphate. |
| References for function | |
| E.C. number | 2.2.1.1 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | Transcriptional regulator
derepresses the marRAB multiple antibiotic resistance operon by binding to the MarR repressor
a "trigger enzyme" |
| References for function | Domain F, Bina XR, Levy SB. Transketolase A, an enzyme in central metabolism, derepresses the marRAB multiple antibiotic resistance operon of Escherichia coli by interaction with MarR. Mol Microbiol. 2007 Oct. PMID: 17850260 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | with DNA |
| Comments | |