| General Information |
| MoonProt ID | 201 |
| First appeared in release | 1.0 |
| Name(s) | Hexokinase-2
Hexokinase PII
Hexokinase-B
Gene Name:HXK2
|
| UniProt ID | P04807 (HXKB_YEAST), Reviewed |
| GO terms | GO:0001302 replicative cell aging
GO:0005975 carbohydrate metabolic process
GO:0006000 fructose metabolic process
GO:0006006 glucose metabolic process
GO:0006013 mannose metabolic process
GO:0006096 glycolytic process
GO:0008152 metabolic process
GO:0016310 phosphorylation
GO:0019318 hexose metabolic process
GO:0032445 fructose import
GO:0046015 regulation of transcription by glucose
GO:0046323 glucose import
GO:0046835 carbohydrate phosphorylation
GO:0000166 nucleotide binding
GO:0003824 catalytic activity
GO:0004396 hexokinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
GO:0005634 nucleus
GO:0005739 mitochondrion
GO:0005829 cytosol |
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae (yeast, fungi) |
| Sequence length | 486 |
| FASTA sequence | >sp|P04807|HXKB_YEAST Hexokinase-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HXK2 PE=1 SV=4
MVHLGPKKPQARKGSMADVPKELMQQIENFEKIFTVPTETLQAVTKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLRVVLVKLGGDRTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQFPQGISEPIPLGFTFSFPASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALINDTTGTLVASYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGKLSDDIPPSAPMAINCEYGSFDNEHVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDMYKQGFIFKNQDLSKFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGINTTVQERKLIRRLSELIGARAARLSVCGIAAICQKRGYKTGHIAADGSVYNRYPGFKEKAANALKDIYGWTQTSLDDYPIKIVPAEDGSGAGAAVIAALAQKRIAEGKSVGIIGA
|
| Structure Information |
| PDB ID | 1IG8 |
| Quaternary structure | |
| SCOP | Ribonuclease H-like motif |
| CATH | 1.10.287.1250, 3.30.420.40, 3.40.367.20 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-19, 253-256, 477-486 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | hexokinase, enzyme
ATP + D-hexose => ADP + D-hexose 6-phosphate.
Carbohydrate metabolism, hexose metabolism, glycolysis |
| References for function | |
| E.C. number | 2.7.1.1 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | Hxk2 interacts directly with Mig1, a transcriptional repressor that binds to promoters of genes that are repressed by glucose
high glucose causes Hxk2 to move to the nucleus |
| References for function | Ahuatzi D, Herrero P, de la, Moreno F. The glucose-regulated nuclear localization of hexokinase 2 in Saccharomyces cerevisiae is Mig1-dependent. J Biol Chem. 2004 Apr 2. PMID: 14715653.
Herrero P, Mart?nezCampa C, Moreno F. The hexokinase 2 protein participates in regulatory DNA-protein complexes necessary for glucose repression of the SUC2 gene in Saccharomyces cerevisiae. FEBS Lett. 1998 Aug 28. PMID: 9738454.
Rodr?guez A, De La, Herrero P, Moreno F. The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae. Biochem J. 2001 May 1. PMID: 11311123. |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | nucleus |
| Comments | |