| General Information |
| MoonProt ID | 203 |
| First appeared in release | 1.0 |
| Name(s) | Pyruvate carboxylase
PCB
Gene Name:PYC |
| UniProt ID | Q8X1T3 (PYC_PICAN), Reviewed |
| GO terms | GO:0006094 gluconeogenesis
GO:0008152 metabolic process
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004075 biotin carboxylase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0046872 metal ion binding
GO:0005737 cytoplasm |
| Organisms for which functions have been demonstrated | Hansenula poymorpha (Pichia angusta) (Yeast) |
| Sequence length | 1175 |
| FASTA sequence | >sp|Q8X1T3|PYC_PICAN Pyruvate carboxylase OS=Pichia angusta GN=PYC PE=3 SV=1
MAQVEDYSSLHRLRKNSEILSNANKILVANRGEIPIRIFRSAHELSMQTVAIYSHEDRLSMHRLKADEAYVIGARGQYSPVQAYLQIDEIINIALEHNVSMIHPGYGFLSENSEFARKVEDSGMIWIGPPHNVIDAVGDKVSARNLAGKCNVPVVPGTDGPIDSVEQAQEFVDKYGYPVIIKAAFGGGGRGMRVVREGESIADAFQRATSEAKTAFGNGTCFIERFLDKPKHIEVQLLADNYGNVIHLFERDCSVQRRHQKVVEIAPAKTLPVEVRDAILTDAVKLAKAANYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEVTGVDIVAAQIQIAAGASLQQLGLLQDKITTRGFAIQCRITTEDPAKNFQPDTGKIEVYRSSGGNGVRLDGGNGFAGAIISPHYDSMLVKCSTSGSNYEIARRKMIRALVEFRIRGVKTNIPFLLALLTHPTFVSGDCWTTFIDDTPSLFEMVQSKNRAQKLLSYLADLCVNGSSIKGQIGLPKLTRDADIPVIHDINGWDIDIKNTPPPESFRQYLLDYGPEQFANQIRAFDGCLIMDTTWRDAHQSLLATRVRTIDLLNIAPATAHAFRYAFALECWGGATFDVAMRFLHEDPWDRLRKLRKAVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDAGVDIFRVFDALNDLEQLKVGVDAVKKAGGVVEATVCYSGDMLKPGKKYNLKYYLETVDKIMEMGTHLLGIKDMAGTLKPAAAKLLISSIRKKYPSVPIHVHTHDSAGTGVITYVACALAGADVVDCAVNSMSGLTSQPSMSAFIAALDNEINTGITEQNAREIDAYWSEMRLLYSCFEADLKGPDPEVYNHEIPGGQLTNLLFQAQQVGLGEKWLETKKAYEEANMLLGDIVKVTPTSKVVGDLAQFMVSNKLSPKDVERLASELDFPDSVLDFFEGLMGTPYGGFPEPLRTNILAGKRRKLTRRPGLELEPFDLKKIKEELQSRFGNSITECDVASYNMYPKVFESFKKIQEKYGDLSVLPTRFFLAPPKLNEEISVEIEQGKTFVIKVMAIGDLSPQTGTREVYFEFNGEMRKVTVEDKLAAVETVTRPKADAHNPNEVGAPMAGVVIEVRVHPGVEVKKGDPLCVLSAMKMEMVISSPVSGRVGEVIVHENDSVDAGDLICKITK |
| Structure Information |
| PDB ID | closest is Listeria with 50% amino acid sequence identity |
| Quaternary structure | |
| SCOP | NA |
| CATH | NA |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Pyruvate carboxylase, enzyme
ATP + pyruvate + HCO3- => ADP + phosphate + oxaloacetate.
Carbohydrate biosynthesis, gluconeogenesis
|
| References for function | |
| E.C. number | 6.4.1.1 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | assembly of peroxisomal alcohol oxidase (AOX)
may mediate FAD binding to AOX monomers in cytoplasm, which enables them to be transported to the peroxisome where they can assemble into the active octomeric form |
| References for function | Ozimek P, van Dijk, Latchev K, Gancedo C, Wang DY, van der, Veenhuis M. Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase. Mol Biol Cell. 2003 Feb. PMID: 12589070.
Ozimek PZ, Klompmaker SH, Visser N, Veenhuis M, van der. The transcarboxylase domain of pyruvate carboxylase is essential for assembly of the peroxisomal flavoenzyme alcohol oxidase. FEMS Yeast Res. 2007 Oct. PMID: 17316367. |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |