| General Information |
| MoonProt ID | 218 |
| First appeared in release | 1.0 |
| Name(s) | Glycogen debranching enzyme
TreX
Gene Name:treX |
| UniProt ID | Q7LX99 (Q7LX99_SULSO), Unreviewed |
| GO terms | GO:0005975 carbohydrate metabolic process
GO:0005980 glycogen catabolic process
GO:0008152 metabolic process
GO:0003824 catalytic activity
GO:0004133 glycogen debranching enzyme activity
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0016787 hydrolase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0043169 cation binding |
| Organisms for which functions have been demonstrated | Sulfologus solfataricus |
| Sequence length | 718 |
| FASTA sequence | >tr|Q7LX99|Q7LX99_SULSO Glycogen debranching enzyme (TreX) OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=treX PE=4 SV=1
MALFFRTRDRPLRPGDPYPLGSNWIEDDDGVNFSLFSENAEKVELLLYSLTNQKYPKEIIEVKNKTGDIWHVFVPGLRPGQLYAYRVYGPYKPELGLRFNPNKVLIDPYAKAINGSVIWNDAVFGYKIGDQNQDLTYDERDSGEYVPKSVVINPYFEWDDEDFIKGKKVPLKDTVIYEVHVKGFTKLRLDLPENIRGTYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLTDKGLTNYWGYDPINFFSPECRYSSTGCLGGQVLSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNTAYYMLQPDNKRYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTEMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAEPWDVGQGGYQVGNFPYQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVSYNQKHNEANGFNNQDGMNENYSWNCGAEGPTNDQNVVICREKQKRNFMITLLVSQGTPMILGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSKFLEFVKKMIQFYRAHPAFRRERYFQGKKLFGMPLKDVTFYTLEGREVDEKTWSSPTQLVIFVLEGSVMDEINMYGERIADDSFLIILNANPNNVKVKFPKGKWELVISSYLREIKPEERIIEGEKELEIEGRTALVYRRIEL
|
| Structure Information |
| PDB ID | 2VNC
2VR5
2VUY |
| Quaternary structure | |
| SCOP | NA |
| CATH | 2.60.40.10, 3.20.20.80, 2.60.40.1180 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-11, 127-140, 489-497, 644-647, 694-697, 716-718 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | alpha-1,4-transferase, enzyme
|
| References for function | Woo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH. Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus. J Biol Chem. 2008 Oct 17. PMID: 18703518 |
| E.C. number | 3.2.1.- |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | "TreX exhibits two different active-site configurations depending on its oligomeric state. The N terminus of one subunit is located at the active site of the other molecule, resulting in a reshaping of the active site in the tetramer. This is accompanied by a large shift in the "flexible loop" (amino acids 399-416), creating connected holes inside the tetramer." from Woo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH. Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus. J Biol Chem. 2008 Oct 17;283(42):28641-8. doi: 10.1074/jbc.M802560200. PMID: 18703518 |
| Function 2 |
| Function description | alpha-1,6-glucosidase, enzyme |
| References for function | Woo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH. Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus. J Biol Chem. 2008 Oct 17. PMID: 18703518 |
| E.C. number | 3.2.1.33 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |