| General Information |
| MoonProt ID | 222 |
| First appeared in release | 1.0 |
| Name(s) | Dihydrofolate reductase
DHFR
Gene Name:DHFR |
| UniProt ID | P00374 (DYR_HUMAN), Reviewed |
| GO terms | GO:0000082 G1/S transition of mitotic cell cycle
GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:0000278 mitotic cell cycle
GO:0006545 glycine biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0006766 vitamin metabolic process
GO:0006767 water-soluble vitamin metabolic process
GO:0009165 nucleotide biosynthetic process
GO:0031427 response to methotrexate
GO:0044281 small molecule metabolic process
GO:0046209 nitric oxide metabolic process
GO:0046653 tetrahydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046655 folic acid metabolic process
GO:0050999 regulation of nitric-oxide synthase activity
GO:0055114 oxidation-reduction process
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0004146 dihydrofolate reductase activity
GO:0008144 drug binding
GO:0016491 oxidoreductase activity
GO:0050661 NADP binding
GO:0005575 cellular_component
GO:0005654 nucleoplasm
GO:0005829 cytosol |
| Organisms for which functions have been demonstrated | Homo sapiens (human, a mammal) |
| Sequence length | 187 |
| FASTA sequence | >sp|P00374|DYR_HUMAN Dihydrofolate reductase OS=Homo sapiens GN=DHFR PE=1 SV=2
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
|
| Structure Information |
| PDB ID | 1MVS,
1MVT,
3FS6,
3GYF,
2W3A,
2W3B,
2W3M,
2M6J,
4M6K,
4M6L,
3F8Y, 3GI2, 1DHF,
2DHF, 1KMS, 1KMV, 1PD8, 1PD9, 1PDB, 1S3U, 1S3V, 1S3W, 1U72, 1YHO, 2C2S, 2C2T, 1C2T, 1DRF, 1HFR, 1OHJ, 1OHK, 3GHW, 3NXR, 3NXT, 3NXO, 3L3R, 1DLR, 1DLS, 1U71, 1HFQ, 3F91, 1BOZ, 3 |
| Quaternary structure | |
| SCOP | Dihydrofolate reductase-like |
| CATH | 3.40.430.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1 to 2 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | dihydrofolate reductase, enzyme
5,6,7,8-tetrahydrofolate + NADP+ => 7,8-dihydrofolate + NADPH
tetrahydrofolate biosynthesis |
| References for function | |
| E.C. number | 1.5.1.3 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds DHFR mRNA
regulation of DHFR synthesis
methotrexate inhibits interaction
|
| References for function | Chu E, Takimoto CH, Voeller D, Grem JL, Allegra CJ. Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger RNA in vitro. Biochemistry. 1993 May 11. PMID: 8490020 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |