| General Information |
| MoonProt ID | 235 |
| First appeared in release | 1.0 |
| Name(s) | Alanyl-tRNA synthetase
Alanine--tRNA ligase
AlaRS
Gene Name: alaS |
| UniProt ID | P00957 (SYA_ECOLI), Reviewed |
| GO terms | GO:0006412 translation
GO:0006419 alanyl-tRNA aminoacylation
GO:0006450 regulation of translational fidelity
GO:0043039 tRNA aminoacylation
GO:0045892 negative regulation of transcription, DNA-templated
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0001141 bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription
GO:0002161 aminoacyl-tRNA editing activity
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004813 alanine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016874 ligase activity
GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0005829 cytosol
GO:0016020 membrane
|
| Organisms for which functions have been demonstrated | Escherichia coli (Gram negative bacterium) |
| Sequence length | 876 |
| FASTA sequence | >sp|P00957|SYA_ECOLI Alanine--tRNA ligase OS=Escherichia coli (strain K12) GN=alaS PE=1 SV=2
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNTQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLGSTIIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVKGWVSAKLQ |
| Structure Information |
| PDB ID | 3HXU
3HXV
3HXW
3HXX
3HXY
3HXZ
3HY0
3HY1 |
| Quaternary structure | |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-7, 435-445, 751-760, 873-76 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Alanyl-tRNA synthetase, enzyme
attaches alanine to tRNA(Ala)
ATP + L-alanine + tRNA(Ala) => AMP + diphosphate + L-alanyl-tRNA(Ala) |
| References for function | |
| E.C. number | 6.1.1.7 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | transcription regulator
represses transcription of its own gene
increased repression with increased alanine concentrations
binds DNA
|
| References for function | Putney SD, Schimmel P. An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription. Nature. 1981 Jun 25. PMID: 6264314 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | binding to DNA |
| Comments | |