| General Information |
| MoonProt ID | 236 |
| First appeared in release | 1.0 |
| Name(s) | Leucyl-tRNA synthetase
Leucine--tRNA ligase, mitochondrial
ymLeuRS
LeuRS
Gene Name: NAM2 |
| UniProt ID | P11325 |
| GO terms | GO:0000372 Group I intron splicing
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006429 leucyl-tRNA aminoacylation
GO:0006450 regulation of translational fidelity
GO:0032543 mitochondrial translation
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0003729 mRNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004823 leucine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
|
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae (yeast, fungi) |
| Sequence length | 894 |
| FASTA sequence | >sp|P11325|SYLM_YEAST Leucine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAM2 PE=1 SV=1
MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTLNSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIAKMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLKDWPSNVKQMQKNWIGESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVALALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTIDEFVNVACPSCGSPAKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADALNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDFQRFLKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNEMIVQNLQKLVTVIYPAVPSISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGRVKFMYTADKNFLKLGRDAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK
|
| Structure Information |
| PDB ID | |
| Quaternary structure | |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-17, 37-45, 381-391, 625-636, 712-727, 893-894 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Leucyl-tRNA synthetase, enzyme
ATP + L-leucine + tRNA(Leu) => AMP + diphosphate + L-leucyl-tRNA(Leu)
protein synthesis |
| References for function | |
| E.C. number | 6.1.1.4 |
| Location of functional site(s) | |
| Cellular location of function | Mitochondrial matrix. |
| Comments | |
| Function 2 |
| Function description | intron splicing, RNA splicing
group I intron splicing
|
| References for function | Sarkar J, Poruri K, Boniecki MT, McTavish KK, Martinis SA. Yeast mitochondrial leucyl-tRNA synthetase CP1 domain has functionally diverged to accommodate RNA splicing at expense of hydrolytic editing. J Biol Chem. 2012 Apr 27. PMID: 22383526 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | nucleus |
| Comments | |