| General Information |
| MoonProt ID | 239 |
| First appeared in release | 1.0 |
| Name(s) | Lysyl-tRNA synthetase
Lysine-tRNA ligase
Gene Name: Kars |
| UniProt ID | Q5XIM7 (Q5XIM7_RAT), Unreviewed |
| GO terms | GO:0006418 tRNA aminoacylation for protein translation
GO:0006430 lysyl-tRNA aminoacylation
GO:0015966 diadenosine tetraphosphate biosynthetic process
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004824 lysine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016597 amino acid binding
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0015630 microtubule cytoskeleton
GO:0017101 aminoacyl-tRNA synthetase multienzyme complex |
| Organisms for which functions have been demonstrated | Rattus norvegicus (rat, a mammal) |
| Sequence length | 626 |
| FASTA sequence | >tr|Q5XIM7|Q5XIM7_RAT Lysine--tRNA ligase OS=Rattus norvegicus GN=Kars PE=2 SV=1
MLMQAAVRLVRGSLRQTSWAEWGQRELRLGQLAPFTTLRKDKPLSDRRSELKRRLKAEKKLAEKEAKQKELSEKQLNQTTAAAATNHTADNGVGAEEETLDPNQYYKIRSQAVQQLKVSGEDPYPHKFHVDISLTQFIQEYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFVHINNKLRRGDIIGVEGNPGKTKKGELSIVPREMTLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMLSGMVRSITGSYKITYHPDGPEGQAYEIDFTPPFRRISMVEELEKVLGVKLPETSLFETEETRKILDDICVARAVECPPPRTTARLLDKLVGEFLEVTCISPTFICDHPQIMSPLAKWHRCKEGLTERFELFVMKKEICNAYTELNDPVRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVTMFLTDSNNIKEVLLFPAMKPEDKKETATATETPESTEASPSV
|
| Structure Information |
| PDB ID | |
| Quaternary structure | |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-13, 41-98, 605-626 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Lysyl-tRNA synthetase, enzyme
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
protein synthesis |
| References for function | |
| E.C. number | 6.1.1.6 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | synthesis of diadenosine tetraphosphate (diadenosine-5',5'''-P1,P4-tetraphosphate, Ap(4)A, AppppA)
Ap4A then regulates transcription of MITF target genes
|
| References for function | Wahab SZ, Yang DC. Synthesis of diadenosine 5',5''' -P1,P4-tetraphosphate by lysyl-tRNA synthetase and a multienzyme complex of aminoacyl-tRNA synthetases from rat liver. J Biol Chem. 1985 May 10. PMID: 3988754.
YannayCohen N, CarmiLevy I, Kay G, Yang CM, Han JM, Kemeny DM, Kim S, Nechushtan H, Razin E. LysRS serves as a key signaling molecule in the immune response by regulating gene expression. Mol Cell. 2009 Jun 12. PMID: 19524539.
YannayCohen N, Razin E. Translation and transcription: the dual functionality of LysRS in mast cells. Mol Cells. 2006 Oct 31. PMID: 17085962. |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | nucleus |
| Comments | |