| General Information |
| MoonProt ID | 244 |
| First appeared in release | 1.0 |
| Name(s) | Lysyl-tRNA synthetase
Lysine-tRNA ligase
LysRS
Gene Name: KARS |
| UniProt ID | Q15046 (SYK_HUMAN), Reviewed |
| GO terms | GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006430 lysyl-tRNA aminoacylation
GO:0008033 tRNA processing
GO:0008219 cell death
GO:0010467 gene expression
GO:0015966 diadenosine tetraphosphate biosynthetic process
GO:0016032 viral process
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004824 lysine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016597 amino acid binding
GO:0016874 ligase activity
GO:0046872 metal ion binding
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0017101 aminoacyl-tRNA synthetase multienzyme complex |
| Organisms for which functions have been demonstrated | Homo sapiens (human, a mammal) |
| Sequence length | 597 |
| FASTA sequence | >sp|Q15046|SYK_HUMAN Lysine--tRNA ligase OS=Homo sapiens GN=KARS PE=1 SV=3
MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV
|
| Structure Information |
| PDB ID | 4DPG
3BJU |
| Quaternary structure | |
| SCOP | OB-fold, Class II aaRS and biotin synthetases |
| CATH | 2.40.50.140, 3.30.930.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-18, 21-69, 114-116, 577-597 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Lysyl-tRNA synthetase, enzyme
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
protein synthesis
|
| References for function | |
| E.C. number | 6.1.1.6 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | cytokine
binds to macrophages and peripheral blood mononuclear cells
increases TNF-alpha production by target cells
increases target cell migration
|
| References for function | Park SG, Kim HJ, Min YH, Choi EC, Shin YK, Park BJ, Lee SW, Kim S. Human lysyl-tRNA synthetase is secreted to trigger proinflammatory response. Proc Natl Acad Sci U S A. 2005 May 3. PMID: 15851690 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | extracellular, secreted |
| Comments | |