| General Information |
| MoonProt ID | 324 |
| First appeared in release | 2.0 |
| Name(s) | eIF3 subunit A, Eukaryotic translation initiation factor 3 subunit A
Gene: EIF3A |
| UniProt ID | Q14152 (EIF3A_HUMAN) |
| GO terms | GO:0001731 formation of translation preinitiation complex
GO:0001732 formation of cytoplasmic translation initiation complex
GO:0006412 translation
GO:0006413 translational initiation
GO:0006446 regulation of translational initiation
GO:0010467 gene expression
GO:0044267 cellular protein metabolic process
GO:0003743 translation initiation factor activity
GO:0005198 structural molecule activity
GO:0005515 protein binding
GO:0044822 poly(A) RNA binding
GO:0003743 translation initiation factor activity
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005852 eukaryotic translation initiation factor 3 complex
GO:0016020 membrane
GO:0016282 eukaryotic 43S preinitiation complex
GO:0033290 eukaryotic 48S preinitiation complex
GO:0071541 eukaryotic translation initiation factor 3 complex, eIF3m |
| Organisms for which functions have been demonstrated | Homo sapiens (human, a mammal) |
| Sequence length | 1382 amino acids |
| FASTA sequence | >gi|4503509|ref|NP_003741.1| eukaryotic translation initiation factor 3 subunit A [Homo sapiens]
MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR |
| Structure Information |
| PDB ID | 3J8B partial |
| Quaternary structure | part of 13 subunits (a-m) complex, 800 kilodaltons |
| SCOP | NA |
| CATH | NA |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-10, 103-117, 136-138, 213-214, 307-316, 333-315, 493-564, 569-652, 676-694, 723-741, 774-1382 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | part of eIF3 13-subunit complex that organizes initiation factor and ribosome interactions required for productive translation, protein scaffold for formation of initiation complexes, binds to proteins of small ribosomal subunit |
| References for function | 20094052 |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | |
| Comments | overall complex is phosphorylated: Andaya A, Villa N, Jia W, Fraser CS, Leary JA. Phosphorylation stoichiometries of human eukaryotic initiation factors. Int J Mol Sci. 2014 Jun 27;15(7):11523-38. doi: 10.3390/ijms150711523. PMID: 24979134 |
| Function 2 |
| Function description | binds to 5' untranslated regions of specific mRNA for translational activation, subunits a, b, d, g, binding site on mRNA for c-Jun is required for efficient translation of c-JUN |
| References for function | Lee AS, Kranzusch PJ, Cate JH. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression. Nature. 2015 Jun 4;522(7554):111-4. doi: 10.1038/nature14267. PMID: 25849773 |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | |
| Comments | binds mRNA stem-loops, with different modes of stem-loop binding to affect translation activation or repression, binds directly to c-JUN mRNA |