| General Information |
| MoonProt ID | 353 |
| First appeared in release | 2.0 |
| Name(s) | glycyl-tRNA synthetase, GlyRS, Glycine--tRNA ligase, Gene: GARS |
| UniProt ID | P41250 (SYG_HUMAN) |
| GO terms | GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006426 glycyl-tRNA aminoacylation
GO:0015966 diadenosine tetraphosphate biosynthetic process
GO:0000166 nucleotide binding
GO:0004081 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004820 glycine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016874 ligase activity
GO:0046983 protein dimerization activity
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0005829 cytosol
GO:0030141 secretory granule
GO:0030424 axon
GO:0042995 cell projection
GO:0070062 extracellular exosome |
| Organisms for which functions have been demonstrated | Homo sapiens (human, a mammal) |
| Sequence length | 739 amino acids |
| FASTA sequence | >sp|P41250|SYG_HUMAN Glycine--tRNA ligase OS=Homo sapiens GN=GARS PE=1 SV=3
MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE |
| Structure Information |
| PDB ID | 2PME, 2Q5H, 2PMF, 4KQE, 2Q5I, 4KR2, 4KR3, 4QEI |
| Quaternary structure | |
| SCOP | |
| CATH | 3.30.930.10, 3.30.40.230, 3.40.50.800 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | Predicted disorder at N terminus (aa 1-5), and C terminus (aa 730-739) |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | glycyl-tRNA synthetase, catalyze the formation of glycyl-tRNAGly as a substrate for ribosomal protein synthesis, P41250 (SYG_HUMAN), ATP + glycine + tRNA(Gly) => AMP + diphosphate + glycyl-tRNA(Gly) AND
P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O => GTP + GMP |
| References for function | |
| E.C. number | 3.6.1.17 AND 6.1.1.14 (two step process) |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds to NEDD8, E1, and E2 (Ubc12) in neddylation pathway, binds the APPBP1 subunit of E1 and captures and protects (like a chaperone) activated E2 (NEDD8-conjugated Ubc12) before the activated E2 reaches a downstream target |
| References for function | 27348078 |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |