| General Information |
| MoonProt ID | 354 |
| First appeared in release | 2.0 |
| Name(s) | glycyl tRNA synthetase, ****NEOMORPHIC MOONLIGHTING PROTEIN***, Glycine--tRNA ligase, Gene: Gars |
| UniProt ID | Q9CZD3 (SYG_MOUSE |
| GO terms | GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006426 glycyl-tRNA aminoacylation
GO:0015966 diadenosine tetraphosphate biosynthetic process
GO:0000166 nucleotide binding
GO:0004081 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004820 glycine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016874 ligase activity
GO:0046983 protein dimerization activity
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0030141 secretory granule
GO:0030424 axon
GO:0042995 cell projection
GO:0070062 extracellular exosome |
| Organisms for which functions have been demonstrated | Mus musculus (Mouse, mammal) |
| Sequence length | 729 amino acids |
| FASTA sequence | >sp|Q9CZD3|SYG_MOUSE Glycine--tRNA ligase OS=Mus musculus GN=Gars PE=1 SV=1
MPCLLPSLLRATRAALPLLSPPRVVAASASQRLLSAPAQPAASRSSMDSAEELLAPLRLAVRQQGDFVRKLKEDKAPQVDVDRAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQAWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPTEKDHPKFQSVADLCLYLYSAKAQVTGQSARKMRLGDAVEQGVINNSVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNKGAVGKAYKKDAKLVLEYLSACDECYISEMELLLSEKGEFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVVPSVIEPSFGLGRIMYTILEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRNGVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAEVSELPNVVRDLANGNITWADVEARYPLFEGQETGKKETVEE |
| Structure Information |
| PDB ID | |
| Quaternary structure | Dimer |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-52, 67-82, 94-107, 215-239, 466-467, 718-729 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | glycyl tRNA synthetase, catalyzes attachment of glycine to its cognate tRNA, ATP + glycine + tRNA(Gly) => AMP + diphosphate + glycyl-tRNA(Gly) AND P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O => GTP + GMP |
| References for function | |
| E.C. number | 3.6.1.17 AND 6.1.1.14 two steps |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | ****NEOMORPHIC MOONLIGHTING PROTEIN***, mutant forms have protein binding activity, bind to neuropilin 1 (Nrp1) recpeptor, directly antagonizes binding of the ligand VEGF (vascular endothelial growth factor) to Nrp1, which is an essential signaling pathway in survival of motor neuron |
| References for function | He W, Bai G, Zhou H, Wei N, White NM, Lauer J, Liu H, Shi Y, Dumitru CD, Lettieri K, Shubayev V, Jordanova A, Guergueltcheva V, Griffin PR, Burgess RW, Pfaff SL, Yang XL. CMT2D neuropathy is linked to the neomorphic binding activity of glycyl-tRNA synthetase. Nature. 2015 Oct 29;526(7575):710-4. doi: 10.1038/nature15510. PMID: 26503042 |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | |
| Comments | mutations result in Charcot-Marie-Tooth disease (CMT type 2D, CMT2D), peripheral neuropathy, weakness and atrophy in hands and feet |