| General Information |
| MoonProt ID | 393 |
| First appeared in release | 2.0 |
| Name(s) | threonyl aminoacyl-tRNA synthetase, TARS, Threonine-tRNA ligase, cytoplasmic, Gene:TARS |
| UniProt ID | P26639 (SYTC_HUMAN) |
| GO terms | GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds
GO:0042803 protein homodimerization activity
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0015629 actin cytoskeleton
GO:0070062 extracellular exosome |
| Organisms for which functions have been demonstrated | Homo sapiens (human, a mammal) |
| Sequence length | 723 amino acids |
| FASTA sequence | >sp|P26639|SYTC_HUMAN Threonine--tRNA ligase, cytoplasmic OS=Homo sapiens GN=TARS PE=1 SV=3
MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGRAELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTPYQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHIMGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFERLEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALKIHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYFFHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHYSENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF |
| Structure Information |
| PDB ID | 4HWT, 4TTV, 4P3N |
| Quaternary structure | |
| SCOP | Anticodon-binding domain-like, Class II aaRS and biotin synthetases |
| CATH | 3.30.930.10, 3.40.50.800 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-46, 75-77, 712-723 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | threonyl aminoacyl-tRNA synthetase, attaches threonine to tRNA, early step in protein synthesis, ATP + L-threonine + tRNA(Thr) -> AMP + diphosphate + L-threonyl-tRNA(Thr) |
| References for function | |
| E.C. number | 6.1.1.3 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | promotes vascular development, demostrated this is separate from role in protein synthesis |
| References for function | 26271225 |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | extracellular |
| Comments | secreted from endothelial cells in response to TNF-? and VEGF, association between TARS expression and advancing stage of ovarian cancer, mutation that prevents synthetase activity is still active in promoting vascular development, single amino acid substitutions in TARS can block the anti-angiogenic effects of BC194, indicating that TARS is the specific target of BC194 |