| General Information |
| MoonProt ID | 412 |
| First appeared in release | 3.0 |
| Name(s) | RING-type E3 ubiquitin transferase BRCA1; gene: brca1.L |
| UniProt ID | Q90X96 |
| GO terms | GO:0004842 ubiquitin-protein transferase activity
GO:0006281 DNA repair
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
GO:0003677 DNA binding
GO:0005634 nucleus
GO:0006974 cellular response to DNA damage stimulus
GO:0046872 metal ion binding
GO:0005694 chromosome
GO:0007049 cell cycle
GO:0005515 protein binding
GO:0005694 chromosome
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0016567 protein ubiquitination
|
| Organisms for which functions have been demonstrated | Xenopus laevis (African clawed frog) |
| Sequence length | 1,579 amino acids |
| FASTA sequence | >tr|Q90X96|Q90X96_XENLA RING-type E3 ubiquitin transferase BRCA1 OS=Xenopus laevis OX=8355 GN=brca1.L PE=2 SV=1
MTCSRMDIEGICSVISVMQKNLECPICLELMKEPVATKCDHIFCKFCMLQLLSKKKKGTVPCPLCKTEVTRRSLQESHRFKLLVEGQLKIIKAFEFDSGYKFFPSQEHTKGLDSTIEDVLVKEDQSIVHCKGYRNRKKGVFNRKTYEETGMLSVSKAEEQFAKEVTRLIPCRQKKPKKEAALIFSNCVPDSSDGDLLNKENGLRNDCSPLHYEKEDTQIPEMEEMVESDLAECEFAESAGSNLLGFDGPEGIPEISAETSINAAGNCDFYGRKTEQFPNDHHCSFKQNIADAEQNKRNQHCGNVPFAPMGKSNLDEKETVETDFDNQHNDSNPENNDPLGKVTKLMRRSTERVNEWLLKTNQDFSTLSAEEDPILDALALQNKETSDKRSCSSDDSELMPVLHKHAEKGISGGGFDKPAVGVKDKIFCKVYKRERKAMPPNNITCVAEVHHDSALETGKENMTLEYGTGMSHLMSKRKMVYSLNPENTSKKNDLANINGSINVFPDCISDANLELEDKSEADSNSADACQSDIVHSSNTQIKQQCVESLANAGETRKKQELSCERSQEEKDFTQSGALGPKTRSQKSPYGHSELHIESSQISNEPSNVTKQVEVRRSRRLLMLPKGPGNKSNSNAVKEMNEQENIAQIPEFRVKDTNKNNEISDTVPSQLKKKDTYFPPCTSEDTGELENGIPVRKISDQNASLDNPIDPCKDEYSDTLLHVAGDNVQPDYMETEESELETQHIVKMFKTSKRTSFILESKEAENENRVNSAVEISQVETSNELPNVAECRHTSLLSSAKEQSGALLKQGSPSSEPKKTSPIHMLKKTESKHSKMSRNRRGKVKPSSNSAKNTTGQPDNLNNPTQGVTGSLYNKQVMSDFPMRLNVDEEHTNASAKGSQSSVADKSTAHSNVNTDFISDINSATPDGLLHYMDKAEGNSSPWDTTRDKNAALLESCLPSAYGSTGEKTPIPKVQSSEEGSSQGLFNQKPKCSSSGKADQKNSSKNPGKSQCRILSDFSGSSNNGKCSSQEMLSKAAFPSLSQESQCSVSLFSTQSNMSQQSVDEDHKQDVLQDELVSPNKTKRTAISGTEETQISPLRDQYQNPDIDEASECESEASHTGDSSILSSQDELLNTQQRNYMKDSLKKLQQEMAALEAVLGQHGTQKLEAETTCIPSSEHVTEMQEATEEEEEETYQGENLFVKTPPEHVANGSTLIGQSTDNGLQGRNRPVSPSFLCQTRIEKDTPEIVCPAMGISKKSLL
TQKELPSQHKQEIRPDLETVNVTPTNPIDAQESLRQSSKNKRGSRMLHNKRSTSNQGSFCLSVEAAESPQIPKQNKAEFGIARKSTSPTFASPARAKVPSVGFKSPVVSSRRNLSFVASGLNQCEMALVQRFSKTTQSILSSRITDSTTHVIMKTDAELVCERTLKYFQGIASRKWVVSYEWVVQSFREGQILDEYDFEVKGDVINGRNHRGPRRSRLGSDGLLLIDFEICFFGSFTDMTLDDLEWMVSECGATVVKKLQFFKKKHNVTSLVIVQPDASTEVRDYTEIRKKHKALVVTREWLMDSVATYRLQKFDAYLA |
| Structure Information |
| PDB ID | NA |
| Quaternary structure | NA |
| SCOP | NA |
| CATH | NA |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | Use FASTA sequence on the MFDp2 webserver.
moonID_412_uniID_Q90X96 is 1579 residues long, with 886 residues (56.11%) predicted as disordered.The protein has 1 short (< 30 residues) disorder segment and 6 long (>= 30 residues) disorder segments.
Segment 1 - Long (>= 30 residues) disordered segment Segment is located between positions 205 and 235 in the sequence. The segment is 31 residues long (1.96 % of the total sequence length).
Segment 2 - Long (>= 30 residues) disordered segment Segment is located between positions 281 and 351 in the sequence. The segment is 71 residues long (4.50 % of the total sequence length).
Segment 3 - Short (< 30 residues) disordered segment Segment is located between positions 371 and 388 in the sequence. The segment is 18 residues long (1.14 % of the total sequence length).
Segment 4 - Long (>= 30 residues) disordered segment Segment is located between positions 515 and 717 in the sequence. The segment is 203 residues long (12.86 % of the total sequence length).
Segment 5 - Long (>= 30 residues) disordered segment Segment is located between positions 751 and 915 in the sequence. The segment is 165 residues long (10.45 % of the total sequence length).
Segment 6 - Long (>= 30 residues) disordered segment Segment is located between positions 933 and 1139 in the sequence. The segment is 207 residues long (13.11 % of the total sequence length).
Segment 7 - Long (>= 30 residues) disordered segment Segment is located between positions 1172 and 1362 in the sequence. The segment is 191 residues long (12.10 % of the total sequence length). |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | physically interacts with BARD1 and spindle-associated proteins TPX2, NuMa, and XRHAMM, required for spindle formation induced by nuclei in cytoplasmic extracts from Xenopus laevis eggs |
| References for function | 17081976 |
| E.C. number | NA |
| Location of functional site(s) | NA |
| Cellular location of function | spindle poles in cytoplasm |
| Comments | required for spindle formation induced by nuclei in cytoplasmic extracts from Xenopus laevis eggs; immunodepletion of BRCA1 results in abnormal morphology of spindles with unfocused poles and higher MT density compared to non-depleted extracts; studies suggest that BRCA1 directly contribute to centrosome-independent spindle assembly |
| Function 2 |
| Function description | RING-type E3 ubiquitin transferase BRCA1 |
| References for function | 23747565 |
| E.C. number | NA |
| Location of functional site(s) | NA |
| Cellular location of function | nucleus |
| Comments | NA |