| General Information |
| MoonProt ID | 463 |
| First appeared in release | 3.0 |
| Name(s) | glyceraldehyde-3-phosphate dehydrogenase |
| UniProt ID | P25858 (G3PC1_ARATH) |
| GO terms | GO:0006979 protein binding
GO:0042542 oxidation-reduction process
GO:0048046 nucleus
GO:0051775 cytoplasm
GO:0006096 DNA binding
GO:0006979 glycolytic process
GO:0009744 oxidoreductase activity
GO:0048316 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
|
| Organisms for which functions have been demonstrated | Arabidopsis thaliana (Mouse-ear cress, a plant) |
| Sequence length | 338 amino acids |
| FASTA sequence | >sp|P25858|G3PC1_ARATH Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic OS=Arabidopsis thaliana OX=3702 GN=GAPC1 PE=1 SV=2
MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEGKLKGILGYTEDDVVSTDFVGDNRSSIFDAKAGIALSDKFVKLVSWYDNEWGYSSRVVDLIVHMSKA |
| Structure Information |
| PDB ID | 4Z0H, 6QUN, 6QUQ |
| Quaternary structure | NA |
| SCOP | |
| CATH | 2 matching superfamilies.
Superfamily: 3.30.360.10 Dihydrodipicolinate Reductase; domain 2.
Superfamily: 3.40.50.720 NAD(P)-binding Rossmann-like Domain.
4 matching domains. Domain: 4z0hO01 PDB code 4z0h, chain O, domain 01 Superfamily: 3.40.50.720
Domain: 4z0hO02 PDB code 4z0h, chain O, domain 02 Superfamily: 3.30.360.10
Domain: 4z0hR01 PDB code 4z0h, chain R, domain 01 Superfamily: 3.40.50.720
Domain: 4z0hR02 PDB code 4z0h, chain R, domain 02 Superfamily: 3.30.360.10 |
| TM Helix Prediction | no TM helices
# sp|P25858|G3PC1_ARATH Number of predicted TMHs: 0
# sp|P25858|G3PC1_ARATH Exp number of AAs in TMHs: 0.02816
# sp|P25858|G3PC1_ARATH Exp number, first 60 AAs: 0.00455
# sp|P25858|G3PC1_ARATH Total prob of N-in: 0.05273
sp|P25858|G3PC1_ARATH TMHMM2.0 outside 1 338 |
| DisProt Annotation | |
| Predicted Disorder Regions | Use FASTA sequence on MFDp2 webserver.
spP25858G3PC1_ARATH_Glyc is 338 residues long, with 0 residues (0.00 %) predicted as disordered. The protein has 0 short (< 30 residues) disorder segments and 0 long (>= 30 residues) disorder segments. |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | glyceraldehyde-3-phosphate dehydrogenase |
| References for function | Rius SP, Casati P, Iglesias AA, Gomez-Casati DF. Characterization of Arabidopsis lines deficient in GAPC-1, a cytosolic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase. Plant Physiol. 2008 Nov;148(3):1655-67. doi: 10.1104/pp.108.128769. Epub 2008 Sep 26. PMID: 18820081; PMCID: PMC2577239. |
| E.C. number | 1.2.1.12 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | Binds NF-YC10 (nuclear factor Y subunit C10).
GAPC interacts with a transcription factor to promote the expression of heat-inducible genes and heat tolerance in Arabidopsis.
Screening of Arabidopsis transcription factors identifies nuclear factor Y subunit C10 (NF-YC10) as a GAPC-binding protein. |
| References for function | Kim SC, Guo L, Wang X. Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress. Nat Commun. 2020 Jul 10;11(1):3439. doi: 10.1038/s41467-020-17311-4. PMID: 32651385; PMCID: PMC7351759. |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | nucleus |
| Comments | |