| General Information |
| MoonProt ID | 481 |
| First appeared in release | 3.0 |
| Name(s) | NAD-dependent protein deacetylase sirtuin-1; Sir-1 |
| UniProt ID | Q96EB6 (SIR1_HUMAN) |
| GO terms | GO:0006476 protein deacetylation
GO:0034979 NAD-dependent protein deacetylase activity
GO:0043433 negative regulation of DNA-binding transcription factor activity
GO:0008134 transcription factor binding
GO:0033558 protein deacetylase activity
GO:1901984 negative regulation of protein acetylation
GO:0010629 negative regulation of gene expression
GO:0090400 stress-induced premature senescence
GO:2000619 negative regulation of histone H4-K16 acetylation
GO:0071441 negative regulation of histone H3-K14 acetylation
GO:0070914 UV-damage excision repair
GO:0002039 p53 binding
GO:0005637 nuclear inner membrane
GO:0005654 nucleoplasm
GO:0005720 nuclear heterochromatin |
| Organisms for which functions have been demonstrated | Homo sapiens (Human) |
| Sequence length | 747 amino acids |
| FASTA sequence | >sp|Q96EB6|SIR1_HUMAN NAD-dependent protein deacetylase sirtuin-1 OS=Homo sapiens OX=9606 GN=SIRT1 PE=1 SV=2
MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS |
| Structure Information |
| PDB ID | 4I5I, chain A/B, aa 241-516 |
| Quaternary structure | NA |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices
# sp|Q96EB6|SIR1_HUMAN Number of predicted TMHs: 0
# sp|Q96EB6|SIR1_HUMAN Exp number of AAs in TMHs: 0.00329
# sp|Q96EB6|SIR1_HUMAN Exp number, first 60 AAs: 0
# sp|Q96EB6|SIR1_HUMAN Total prob of N-in: 0.00024
sp|Q96EB6|SIR1_HUMAN TMHMM2.0 outside 1 747 |
| DisProt Annotation | |
| Predicted Disorder Regions | Use FASTA sequence on the MFDp2 webserver.
sp_Q96EB6_SIR1_HUMAN_NAD is 747 residues long, with 323 residues (43.24 %) predicted as disordered. The protein has 1 short (< 30 residues) disorder segment and 3 long (>= 30 residues) disorder segments. Protein was not aligned to any disorder regions of our templates..
Segment 1 - Long (>= 30 residues) disordered segment
Segment is located between positions 1 and 144 in the sequence.
The segment is 144 residues long (19.28 % of the total sequence length).
Segment 2 - Long (>= 30 residues) disordered segment
Segment is located between positions 509 and 628 in the sequence.
The segment is 120 residues long (16.06 % of the total sequence length).
Segment 3 - Long (>= 30 residues) disordered segment
Segment is located between positions 657 and 698 in the sequence.
The segment is 42 residues long (5.62 % of the total sequence length).
Segment 4 - Short (< 30 residues) disordered segment
Segment is located between positions 731 and 747 in the sequence.
The segment is 17 residues long (2.28 % of the total sequence length).
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| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | deacetylase |
| References for function | Langley E, Pearson M, Faretta M, Bauer UM, Frye RA, Minucci S, Pelicci PG, Kouzarides T. Human SIR2 deacetylates p53 and antagonizes PML p53 induced cellular senescence. The EMBO journal. 2002 May 15;21(10):2383-96. |
| E.C. number | 2.3.1.286 |
| Location of functional site(s) | His363 |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | transcription regulation |
| References for function | Schug TT, Li X. Sirtuin 1 in lipid metabolism and obesity. Annals of medicine. 2011 May 1;43(3):198-211. |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | regulate fatty acid oxidation in the liver, sense nutrient availability in the hypothalamus, influence obesity-induced inflammation in macrophages, and modulate the activity of the circadian clock in metabolic tissues |