| General Information |
| MoonProt ID | 482 |
| First appeared in release | 3.0 |
| Name(s) | Poly [ADP-ribose] polymerase 1; PARP1 |
| UniProt ID | P09874 (PARP1_HUMAN) |
| GO terms | GO:2001170 negative regulation of ATP biosynthetic process
GO:0001228 DNA-binding transcription activator activity
GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
GO:0045944 positive regulation of transcription by RNA polymerase II
GO:0005730 nucleolus
GO:1990404 protein ADP-ribosylase activity
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0006302 double-strand break repair
GO:0070212 protein poly-ADP-ribosylation
GO:0140294 NAD DNA ADP-ribosyltransferase activity
GO:0070213 protein auto-ADP-ribosylation
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0035861 site of double-strand break
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0035861 site of double-strand break
GO:1990966 ATP generation from poly-ADP-D-ribose
GO:0018424 peptidyl-glutamic acid poly-ADP-ribosylation
GO:1905168 positive regulation of double-strand break repair via homologous recombination |
| Organisms for which functions have been demonstrated | Homo sapiens (Human) |
| Sequence length | 1014 amino acids |
| FASTA sequence | >sp|P09874|PARP1_HUMAN Poly [ADP-ribose] polymerase 1 OS=Homo sapiens OX=9606 GN=PARP1 PE=1 SV=4
MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW |
| Structure Information |
| PDB ID | 1UK0, chain A/B, aa 662-1011; 1UK1, chain A/B, aa 662-1011 |
| Quaternary structure | NA |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | |
| Predicted Disorder Regions | Predicted disorder at N terminus (aa 1-7), and middle region (aa 88-100, 140-142, 198-212, 215-219, 359-382, 502-531, 946-955) |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | poly[ADP-ribose]polymerase 1 |
| References for function | de Murcia G, De Murcia JM. Poly (ADP-ribose) polymerase. A molecular nick sensor. Trends Biochem Sci. 1994;19:172-6. |
| E.C. number | 2.4.2.30 |
| Location of functional site(s) | Active sites: Gln763, Gly863, Tyr889, Tyr869, Lys903, Ser904, Tyr907
Skalitzky DJ, Marakovits JT, Maegley KA, Ekker A, Yu XH, Hostomsky Z, Webber SE, Eastman BW, Almassy R, Li J, Curtin NJ. Tricyclic benzimidazoles as potent poly (ADP-ribose) polymerase-1 inhibitors. Journal of medicinal chemistry. 2003 Jan 16;46(2):210-3. |
| Cellular location of function | nucleus |
| Comments | |
| Function 2 |
| Function description | Transcription factor |
| References for function | Langelier MF, Ruhl DD, Planck JL, Kraus WL, Pascal JM. The Zn3 domain of human poly (ADP-ribose) polymerase-1 (PARP-1) functions in both DNA-dependent poly (ADP-ribose) synthesis activity and chromatin compaction. Journal of biological chemistry. 2010 Jun 11;285(24):18877-87. |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | nucleus |
| Comments | With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription |