| General Information |
| MoonProt ID | 584 |
| First appeared in release | 4.0 |
| Name(s) | aspartate aminotransferase Aat2 |
| UniProt ID | P23542 |
| GO terms | GO:0032938 negative regulation of translation in response to oxidative stress
GO:0003824 catalytic activity
GO:0004069 L-aspartate:2-oxoglutarate aminotransferase activity
GO:0008483 transaminase activity
GO:0016740 transferase activity
GO:0030170 pyridoxal phosphate binding
GO:0043023 ribosomal large subunit binding
GO:0006103 2-oxoglutarate metabolic process
GO:0006520 amino acid metabolic process
GO:0006531 aspartate metabolic process
GO:0006532 aspartate biosynthetic process
GO:0006536 glutamate metabolic process
GO:0005829 cytosol
GO:0005737 cytoplasm
GO:0005777 peroxisome
GO:0005777 peroxisome
GO:0005829 cytosol |
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Sequence length | 418.0 |
| FASTA sequence | >sp|P23542|AATC_YEAST Aspartate aminotransferase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AAT2 PE=1 SV=3
MSATLFNNIELLPPDALFGIKQRYGQDQRATKVDLGIGAYRDDNGKPWVLPSVKAAEKLI
HNDSSYNHEYLGITGLPSLTSNAAKIIFGTQSDAFQEDRVISVQSLSGTGALHISAKFFS
KFFPDKLVYLSKPTWANHMAIFENQGLKTATYPYWANETKSLDLNGFLNAIQKAPEGSIF
VLHSCAHNPTGLDPTSEQWVQIVDAIASKNHIALFDTAYQGFATGDLDKDAYAVRLGVEK
LSTVSPVFVCQSFAKNAGMYGERVGCFHLALTKQAQNKTIKPAVTSQLAKIIRSEVSNPP
AYGAKIVAKLLETPELTEQWHKDMVTMSSRITKMRHALRDHLVKLGTPGNWDHIVNQCGM
FSFTGLTPQMVKRLEETHAVYLVASGRASIAGLNQGNVEYVAKAIDEVVRFYTIEAKL |
| Structure Information |
| PDB ID | 1YAA |
| Quaternary structure | homodimer |
| SCOP | 80199301 YAA A:2-411
80323101 YAA A:2-411 |
| CATH | 1yaaA01 , 1yaaA02 , 1yaaB01 , 1yaaB02 , 1yaaC01 , 1yaaC02 , 1yaaD01 , 1yaaD02 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | not in DisProt |
| Predicted Disorder Regions | Predicted disorder N terminus (aa 1-5) and C terminus (aa 417-418). |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | enzyme,asparate aminotransferase, cytoplasmic isoform |
| References for function | Crawford RA, Ashe MP, Hubbard SJ, Pavitt GD. Cytosolic aspartate aminotransferase moonlights as a ribosome-binding modulator of Gcn2 activity during oxidative stress. Elife. 2022 May 27;11:e73466. doi: 10.7554/eLife.73466. PMID: 35621265; PMCID: PMC9191892. |
| E.C. number | EC:2.6.1.1 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds to 60S (large subunit of the ribosome and modulates Gcn2 activation in response to H2O2 stress, |
| References for function | Crawford RA, Ashe MP, Hubbard SJ, Pavitt GD. Cytosolic aspartate aminotransferase moonlights as a ribosome-binding modulator of Gcn2 activity during oxidative stress. Elife. 2022 May 27;11:e73466. doi: 10.7554/eLife.73466. PMID: 35621265; PMCID: PMC9191892. |
| E.C. number | |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |