Protein Information

General Information
MoonProt ID6
First appeared in release1.0
Name(s)Phosphoglucose isomerase PGI GPI AMF NLK Glucose-6-phosphate isomerase Autocrine motility factor Neuroleukin Differentiation and maturation mediator Phosphohexose isomerase PHI
UniProt IDP06745 (G6PI_MOUSE), Reviewed
GO termsGO:0001525 angiogenesis GO:0001701 in utero embryonic development GO:0001707 mesoderm formation GO:0006094 gluconeogenesis GO:0006096 glycolytic process GO:0034101 erythrocyte homeostasis GO:0042593 glucose homeostasis GO:0004347 glucose-6-phosphate isomerase activity GO:0005125 cytokine activity GO:0005515 protein binding GO:0008083 growth factor activity GO:0016853 isomerase activity GO:0005576 extracellular region GO:0005615 extracellular space GO:0005737 cytoplasm GO:0005886 plasma membrane GO:0060170 ciliary membrane
Organisms for which functions have been demonstratedStaphylococcus aureus (Gram positive bacterium, causes staph infections, abcesses/boils)
Sequence length558
Structure Information
Quaternary structureDimer of identical subunits
SCOPSIS domain
CATH3.40.50.10490, 1.10.1390.10
TM Helix Predictionno TM helices
DisProt AnnotationNot in DisProt
Predicted Disorder Regions1-9,449-457,531-534, 553-558
Connections to Disease
Function 1
Function descriptionCatalyzes interconversion of glucose-6-phosphate and fructose-6-phosphate in glycolysis and gluconeogenesis
References for functionLohmann, K. (1933) Biochem. Z. 262, 137. Rose IA, OConnell EL. Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction. J Biol Chem. 1961 Dec;236:3086-92. PMID: 14493830. Rose IA. Mechanism of the aldose-ketose isomerase reactions. Adv Enzymol Relat Areas Mol Biol. 1975;43:491-517. PMID: 1106126. Rose IA. Chemistry of proton abstraction by glycolytic enzymes (aldolase, isomerases and pyruvate kinase). Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):131-43. PMID: .6115413 Willem R, Malaisse-Lagae F, Ottinger R, Malaisse WJ. Phosphoglucoisomerase-catalysed interconversion of hexose phosphates. Kinetic study by 13C n.m.r. of the phosphoglucoisomerase reaction in 2H2O. Biochem J. 1990 Jan 15;265(2):519-24. PMID: 2154192.
E.C. number5.3.1.9
Location of functional site(s)Active site pocket containing His388, Glu357, Arg272, Lys518, Thr214, Ser209, Thr211, Ser159
Cellular location of functionCytoplasm
CommentsMultistep catalytic reaction involves ring opening followed by isomerization step, strict specificity for glucose-6-phosphate and fructose-6-phosphate
Function 2
Function descriptionCytokine/growth factor, binds to target cells and causes pre-B cells to mature into antibody secreting cells, supports survival of embryonal neurons, causes differentiation of some leukemia cell lines
References for functionChaput M, Claes V, Portetelle D, Cludts I, Cravador A, Burny A, Gras H, Tartar A. The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase. Nature 1988 Mar 31;332(6163):454-5. PMID: 3352744. Faik P, Walker JI, Redmill AA, Morgan MJ. Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3' sequences. Nature. 1988 Mar 31;332(6163):455-7. PMID: 3352745. Watanabe H, Takehana K, Date M, Shinozaki T, Raz A. Tumor cell autocrine motility factor is the neuroleukin/phosphohexose isomerase polypeptide. Cancer Res. 1996 Jul 1;56(13):2960-3. PMID: 8674049. Xu W, Seiter K, Feldman E, Ahmed T, Chiao JW. The differentiation and maturation mediator for human myeloid leukemia cells shares homology with neuroleukin or phosphoglucose isomerase. Blood. 1996 Jun 1;87(11):4502-6. PMID: 8639816. Gurney ME, Heinrich SP, Lee MR, Yin HS. Molecular cloning and expression of neuroleukin, a neurotrophic factor for spinal and sensory neurons. Science. 1986 Oct 31;234(4776):566-74. PMID: 3764429. Gurney ME, Apatoff BR, Spear GT, Baumel MJ, Antel JP, Bania MB, Reder AT. Neuroleukin: a lymphokine product of lectin-stimulated T cells. Science. 1986 Oct 31;234(4776):574-81. PMID: 3020690. Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A. Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor. J Biol Chem 1991 Jul 15;266(20):13442-8. PMID: 1649192.
E.C. numberN/A
Location of functional site(s)
Cellular location of functionExtracellular