| General Information |
| MoonProt ID | 81 |
| First appeared in release | 1.0 |
| Name(s) | Malate synthase
Gene Name: glcB |
| UniProt ID | P9WK17 |
| GO terms | GO:0006097 glyoxylate cycle
GO:0006099 tricarboxylic acid cycle
GO:0003824 catalytic activity
GO:0004474 malate synthase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0005737 cytoplasm |
| Organisms for which functions have been demonstrated | Mycobacterium tuberculosis (mycobacterium, causes tuberculosis) |
| Sequence length | 741 aa |
| FASTA sequence | >sp|P9WK17|MASZ_MYCTU Malate synthase G OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=glcB PE=1 SV=1
MTDRVSVGNLRIARVLYDFVNNEALPGTDIDPDSFWAGVDKVVADLTPQNQALLNARDELQAQIDKWHRRRVIEPIDMDAYRQFLTEIGYLLPEPDDFTITTSGVDAEITTTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDVIPETDGAEKGPTYNKVRGDKVIAYARKFLDDSVPLSSGSFGDATGFTVQDGQLVVALPDKSTGLANPGQFAGYTGAAESPTSVLLINHGLHIEILIDPESQVGTTDRAGVKDVILESAITTIMDFEDSVAAVDAADKVLGYRNWLGLNKGDLAAAVDKDGTAFLRVLNRDRNYTAPGGGQFTLPGRSLMFVRNVGHLMTNDAIVDTDGSEVFEGIMDALFTGLIAIHGLKASDVNGPLINSRTGSIYIVKPKMHGPAEVAFTCELFSRVEDVLGLPQNTMKIGIMDEERRTTVNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAGPMVRKGTMKSQPWILAYEDHNVDAGLAAGFSGRAQVGKGMWTMTELMADMVETKIAQPRAGASTAWVPSPTAATLHALHYHQVDVAAVQQGLAGKRRATIEQLLTIPLAKELAWAPDEIREEVDNNCQSILGYVVRWVDQGVGCSKVPDIHDVALMEDRATLRISSQLLANWLRHGVITSADVRASLERMAPLVDRQNAGDVAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILHRRRREFKARAAEKPAPSDRAGDDAAR
|
| Structure Information |
| PDB ID | 1N8I 3S9I 3S9Z 3SAD 3SAZ 3SB0 2GQ3 |
| Quaternary structure | |
| SCOP | TIM beta/alpha-barrel |
| CATH | 3.20.20.360, 2.170.170.11, 1.20.1220.12 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-8, 152-159, 383-389, 506-512, 565-571, 676-681, 723-741 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Malate synthase, enzyme
Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA
Acetyl-CoA + H2O + glyoxylate => malate + CoA
Carbohydrate metabolism; glyoxylate cycle
glyoxylate pathway enables bacteria to assimilate two-carbon
compounds into the TCA cycle
|
| References for function | Harada K, Wolfe RG (Aug 1968). "Malic dehydrogenase. VII. The catalytic mechanism and possible role of identical protein subunits". The Journal of Biological Chemistry. 243 (15): 4131–7. |
| E.C. number | 2.3.3.9 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds fibronectin and laminin and A549 lung epithelial cells |
| References for function | Kinhikar AG, Vargas D, Li H, Mahaffey SB, Hinds L, Belisle JT, Laal S. Mycobacterium tuberculosis malate synthase is a laminin-binding adhesin. Mol Microbiol. 2006 May;60(4):999-1013. PMID: 16677310 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cell surface |
| Comments | |