| General Information |
| MoonProt ID | 112 |
| First appeared in release | 1.0 |
| Name(s) | Intron-encoded DNA endonuclease I-AniI
COB intron protein
mRNA maturase bI1
Gene Name: I-AniI |
| UniProt ID | P03880 (ANI1_EMEND), Reviewed |
| GO terms | GO:0006314 intron homing
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0090305 nucleic acid phosphodiester bond hydrolysis
GO:0003677 DNA binding
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0016787 hydrolase activity
GO:0005739 mitochondrion |
| Organisms for which functions have been demonstrated | Emericella nidulans (Aspergillus nidulans) |
| Sequence length | 488 |
| FASTA sequence | >sp|P03880|ANI1_EMEND Intron-encoded DNA endonuclease I-AniI OS=Emericella nidulans GN=I-AniI PE=1 SV=3
MRILKSHPLLKIVNSYIIDSPQPANLSYLWNFGSLLALCLGIQIVTGVTLAMHYTPSVSEAFNSVEHIMRDVNNGWLVRYLHSNTASAFFFLVYLHIGRGLYYGSYKTPRTLTWAIGTVILIVMMATAFLGYVLPYGQMSLWGATVITNLMSAIPWIGQDIVEFIWGGLYTDEPQCGDVLLKILLNAGKSPILGFAYDLFFIIVLLIGVKIAMTRGKSAGVRSLHTSEASQRLHAGDLTYAYLVGLFEGDGYFSITKKGKYLTYELGIELSIKDVQLIYKIKKILGIGIVSFRKINEIEMVALRIRDKNHLKSFILPIFEKYPMFSNKQYDYLRFRNALLSGIISLEDLPDYTRSDEPLNSIESIINTSYFSAWLVGFIEAEGCFSVYKLNKDDDYLIASFDIAQRDGDILISAIRKYLSFTTKVYLDKTNCSKLKVTSVRSVENIIKFLQNAPVKLLGNKKLQYLLWLKQLRKISRYSEKIKIPSNY
|
| Structure Information |
| PDB ID | 2QOJ 3EH8 |
| Quaternary structure | |
| SCOP | Homing endonuclease-like |
| CATH | 3.10.28.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | mRNA maturase
required for splicing of the intron in the cytochrome b (cobA) gene, which contains the coding sequence for the maturase.
stimulates the intrinsic ribozyme activity of the intron by binding to and stabilizing the three-dimensional structure of the RNA. |
| References for function | Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL. Structural and biochemical analyses of DNA and RNA binding a bifunctional homing endonuclease and group I intron splicing factor. Genes Dev. 2003 Dec 1;17(23):2875-88. PMID: 14633971
Ho Y., Kim S.J., Waring R.B. A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease. (1997). Proc. Natl. Acad. Sci. 94:8994-8999. PMID: 9256423 |
| E.C. number | 3.1.26 or 3.1.27, 6.5.1.3? |
| Location of functional site(s) | |
| Cellular location of function | mitochondrion |
| Comments | |
| Function 2 |
| Function description | homing endonuclease
introduces a double-strand break at a specific location in the cobA gene and helps in the insertion of an intron containing its own coding sequence (group I intron)
|
| References for function | Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL. Structural and biochemical analyses of DNA and RNA binding a bifunctional homing endonuclease and group I intron splicing factor. Genes Dev. 2003 Dec 1;17(23):2875-88. PMID: 14633971
Ho Y., Kim S.J., Waring R.B. A protein encoded by a group I intron in Aspergillus nidulans directly assists RNA splicing and is a DNA endonuclease. (1997). Proc. Natl. Acad. Sci. 94:8994-8999. PMID: 9256423 |
| E.C. number | 3.1.21 or 3.1.22 |
| Location of functional site(s) | |
| Cellular location of function | mitochondrion |
| Comments | |