| General Information |
| MoonProt ID | 137 |
| First appeared in release | 1.0 |
| Name(s) | General control protein GCN4
AAS3
ARG9
Amino acid biosynthesis regulatory protein
Gene Name: GCN4
|
| UniProt ID | P03069 (GCN4_YEAST) Reviewed |
| GO terms | GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001080 nitrogen catabolite activation of transcription from RNA polymerase II promoter
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0008652 cellular amino acid biosynthetic process
GO:0010691 negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels
GO:0045899 positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
GO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter
GO:0001084 TFIID-class binding transcription factor activity
GO:0001135 RNA polymerase II transcription factor recruiting transcription factor activity
GO:0001190 RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription
GO:0001191 RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005515 protein binding
GO:0042802 identical protein binding
GO:0043565 sequence-specific DNA binding
GO:0005634 nucleus |
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae |
| Sequence length | 281 |
| FASTA sequence | >sp|P03069|GCN4_YEAST General control protein GCN4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCN4 PE=1 SV=1
MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER |
| Structure Information |
| PDB ID | 1YSA 1ZTA 2ZTA 4DMD 4DME 3GJP |
| Quaternary structure | |
| SCOP | Parallel coiled-coil |
| CATH | 3.30.160.60 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | 32.38%, 101 to 134; 225 to 281 |
| Predicted Disorder Regions | 1 to 12, 20-35, 59-69, 71, 75-84, 139-213, 237-251, 277-281 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | transcription factor; transcription activator |
| References for function | IA Hope and K Struhl. (1986) Functional Dissection of a Eukaryotic Transcriptional Activator Protein, GCN4 of yeast. Cell. 46, 885-894. |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | nucleus |
| Comments | RNA Binding: Arg3, Lys5, Glu8, Lys10, Glu12, Ser16. Y Nikolaev and K Pervushin. (2012) Structural basis of RNA binding by leucine zipper GCN4. Prot Sci. 21, 667-676.
Dimer forming: Asn16. L Gonzalez, D Woolfson, and T Alber. (1996) Buried polar residues and structural specificity in the GCN4 leucine zipper. Nature Struct. Biol. 3, 1011-1018.
DNA Binding: Asn235, Ala238, Ala239, Ser242, Arg243. TE Ellenberger, CJ Brandi, K Struhl, et al. (1992) The GCN4 Basic Region Leucine Zipper Binds DNA as a dimer of Uninterrupted ? Helices: Crystal Structure of the Protein-DNA Complex. Cell. 71, 1223-1237.
DNA Binding: Arg249. M Okagami, M Uero, K Makino, et al. (1995) Sequence-Specific DNA Binding by Covalently Constrained Peptide Dimers of the Basic Leucine Zipper Protein GCN4. Bioorg & Med Chem. 3, 777-784.
(Planys and animals leucine zipper)dimer forming and DNA binding: Leu5, Val9, Leu12, Asn16, Leu19, Val23, Leu26, Val30. T Alber. (1992) Structure of the leucine zipper. Curr Opin in Gene and Devel. 2, 205-210. |
| Function 2 |
| Function description | ribonuclease |
| References for function | Y Nikolaev, C Deillon, S RK Hoffmann, et al. (2010) The Leucine Zipper Domains of the Transcription Factors GCN4 and c-Jun Have Ribonuclease Activity. Plos One. 5, 1-12. PMID: 20505831 |
| E.C. number | 3.1.27.5 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | Ribonuclease active sites: Glu12, Glu13, Glu58, Ser16, His20, His92
Y Nikolaev, C Deillon, S RK Hoffmann, et al. (2010) The Leucine Zipper Domains of the Transcription Factors GCN4 and c-Jun Have Ribonuclease Activity. Plos One. 5, 1-12. |