| General Information |
| MoonProt ID | 163 |
| First appeared in release | 1.0 |
| Name(s) | glutamine synthetase
Glutamate--ammonia ligase
Gene name: glnA
|
| UniProt ID | P12425 (GLNA_BACSU) Reviewed |
| GO terms | GO:0006542 glutamine biosynthetic process
GO:0006807 nitrogen compound metabolic process
GO:0009399 nitrogen fixation
GO:0000166 nucleotide binding
GO:0003824 catalytic activity
GO:0004356 glutamate-ammonia ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0005737 cytoplasm |
| Organisms for which functions have been demonstrated | Bacillus subtilis |
| Sequence length | 444 |
| FASTA sequence | >gi|349594718|gb|AEP90905.1| glutamine synthetase [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MAKYTREDIEKLVKEENVKYIRLQFTDILGTIKNVEIPVSQLGKALDNKVMFDGSSIEGFVRIEESDMYLYPDLNTFVIFPWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDLGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAPTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAVRSCDDIQTFKLVVKTIARKHGLHATFMPKPLFGVNGSGMHCNLSLFKNGVNAFFDENADLQLSETAKHFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEVRSVDPAANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMSKEERMENGIVDLPATLAEALEEFKSNEVMVKALGEHLFEHFIEAKEIEWDMFRTQVHPWEREQYMSQY |
| Structure Information |
| PDB ID | 4S0R, 4LNF |
| Quaternary structure | |
| SCOP | NA |
| CATH | 3.10.20.70, 3.30.590.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1 to 5, 443 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | glutamine synthetase
catalyzes the synthesis of glutamine from glutamate and ammonia
ATP + L-glutamate + NH3 => ADP + phosphate + L-glutamine |
| References for function | |
| E.C. number | 6.3.1.2 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds to transcription factor TnrA, a global regulator of transcription, and prevents it from binding to DNA |
| References for function | Wray LV, Zalieckas JM, Fisher SH. Bacillus subtilis glutamine synthetase controls gene expression through a protein-protein interaction with transcription factor TnrA. Cell. 2001 Nov 16. PMID: 11719184 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | binding to DNA |
| Comments | |