| General Information |
| MoonProt ID | 167 |
| First appeared in release | 1.0 |
| Name(s) | Arginase
Gene Name:CAR1 |
| UniProt ID | P00812 (ARGI_YEAST), Reviewed |
| GO terms | GO:0000050 urea cycle
GO:0006525 arginine metabolic process
GO:0019547 arginine catabolic process to ornithine
GO:0090368 regulation of ornithine metabolic process
GO:0004053 arginase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016813 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
GO:0090369 ornithine carbamoyltransferase inhibitor activity
GO:0005829 cytosol |
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae (yeast, fungi) |
| Sequence length | 333 |
| FASTA sequence | >gi|171161|gb|AAA34469.1| arginase [Saccharomyces cerevisiae]
METGPHYNYYKNRELSIVLAPFSGGQGKLGVEKGPKYMLKHGLQTSIEDLGWSTELEPSMDEAQFVGKLKMEKDSTTGGSSVMIDGVKAKRADLVGEATKLVYNSVSKVVQANRFPLTLGGDHSIAIGTVSAVLDKYPDAGLLWIDAHADINTIESTPSGNLHGCPVSFLMGLNKDVPHCPESLKWVPGNLSPKKIAYIGLRDVDAGEKKILKDLGIAAFSMYHVDKYGINAVIEMAMKAVHPETNGEGPIMCSYDVDGVDPLYIPATGTPVRGGLTLREGLFLVERLAESGNLIALDVVECNPDLAIHDIHVSNTISAGCAIARCALGETLL |
| Structure Information |
| PDB ID | Closest homologue in PDB is from Leishmania mexicana with 58.57% amino acid sequence identity; 96% query cover |
| Quaternary structure | |
| SCOP | NA |
| CATH | NA |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1 to 10, 74-82, 332-333 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | arginase
use of arginine as nitrogen source
L-arginine + H2O => L-ornithine + urea
Nitrogen metabolism, urea cycle |
| References for function | |
| E.C. number | 3.5.3.1 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds to ornithine transcarbamylase and inactivates it (OTCase, catalyzes first step in arginine biosynthesis), effectors are ornithine and arginine
|
| References for function | Bechet J, Wiame JM. Indication of a specific regulatory binding protein for ornithinetranscarbamylase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1965 Nov 8. PMID: 5866843.
Messenguy F, Wiame J. The control of ornithinetranscarbamylase activity by arginase in Saccharomyces cerevisiae. FEBS Lett. 1969 Apr. PMID: 11946965. |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |