| General Information |
| MoonProt ID | 209 |
| First appeared in release | 1.0 |
| Name(s) | Cytosol aminopeptidase
Aminopeptidase A/I
Leucine aminopeptidase
LAP
Leucyl aminopeptidase
Gene Name:pepA |
| UniProt ID | P68767 (AMPA_ECOLI), Reviewed |
| GO terms | GO:0006276 plasmid maintenance
GO:0006310 DNA recombination
GO:0006351 transcription, DNA-templated
GO:0006508 proteolysis
GO:0019538 protein metabolic process
GO:0031564 transcription antitermination
GO:0042150 plasmid recombination
GO:0043171 peptide catabolic process
GO:0001073 DNA binding transcription antitermination factor activity
GO:0003677 DNA binding
GO:0004177 aminopeptidase activity
GO:0008233 peptidase activity
GO:0008235 metalloexopeptidase activity
GO:0016787 hydrolase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
GO:0005622 intracellular
GO:0005737 cytoplasm |
| Organisms for which functions have been demonstrated | Escherichia coli (Gram negative bacterium) |
| Sequence length | 503 |
| FASTA sequence | >sp|P68767|AMPA_ECOLI Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1
MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLNDTGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFNVPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNVITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDSGGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRPGDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHITGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITAGCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE
|
| Structure Information |
| PDB ID | 1GYT |
| Quaternary structure | |
| SCOP | Macro domain-like, Phosphorylase/hydrolase-like |
| CATH | 3.40.220.10, 3.40.630.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1, 497-503 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | aminopeptidase, enzyme
removes amino-terminal amino acid, preferentially if it is Leu
|
| References for function | Charlier D, Kholti A, Huysveld N, Gigot D, Maes D, ThiaToong TL, Glansdorff N. Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase. J Mol Biol. 2000 Sep 15. PMID: 10970742 |
| E.C. number | 3.4.11.1 and 3.4.11.10 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | transcriptional repressor
binds DNA, binds car operator DNA
represses the carAB operon
|
| References for function | Charlier D, Kholti A, Huysveld N, Gigot D, Maes D, ThiaToong TL, Glansdorff N. Mutational analysis of Escherichia coli PepA, a multifunctional DNA-binding aminopeptidase. J Mol Biol. 2000 Sep 15. PMID: 10970742 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm, binds to DNA |
| Comments | |