| General Information |
| MoonProt ID | 227 |
| First appeared in release | 1.0 |
| Name(s) | Threonyl-tRNA synthetase
Threonine-tRNA ligase
ThrRS
Gene Name:thrS |
| UniProt ID | P0A8M3 (SYT_ECOLI), Reviewed |
| GO terms | GO:0006412 translation
GO:0006417 regulation of translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation
GO:0045947 negative regulation of translational initiation
GO:0000166 nucleotide binding
GO:0000900 translation repressor activity, nucleic acid binding
GO:0003723 RNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004812
GO:0004829 threonine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0016874 ligase activity
GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds
GO:0046872 metal ion binding
GO:0005737 cytoplasm
|
| Organisms for which functions have been demonstrated | Escherichia coli (Gram negative bacterium) |
| Sequence length | 642 |
| FASTA sequence | >sp|P0A8M3|SYT_ECOLI Threonine--tRNA ligase OS=Escherichia coli (strain K12) GN=thrS PE=1 SV=1
MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGELVDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGFYYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSILDENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNIPFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEVIEKLQQEIRSRSLKQLEE
|
| Structure Information |
| PDB ID | 1QF6
4HWO
4HWP
4HWR
4HWS
1EVK
1EVL
1FYF
1KOG |
| Quaternary structure | |
| SCOP | Anticodon-binding domain-like, beta-Grasp (ubiquitin-like), RRF/tRNA synthetase additional domain-like, Class II aaRS and biotin synthetases |
| CATH | 3.10.20.30, 3.30.980.10, 3.30.54.20, 3.30.930.10, 3.40.50.800 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 633-642 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Threonine-tRNA ligase, enzyme
ATP + L-threonine + tRNA(Thr) => AMP + diphosphate + L-threonyl-tRNA(Thr) |
| References for function | |
| E.C. number | 6.1.1.3 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | binds mRNA
binds mRNA encoding threonyl-tRNA synthetase, controls expression of its own gene at the translational level
|
| References for function | Springer M, Plumbridge JA, Butler JS, Graffe M, Dondon J, Mayaux JF, Fayat G, Lestienne P, Blanquet S, GrunbergManago M. Autogenous control of Escherichia coli threonyl-tRNA synthetase expression in vivo. J Mol Biol. 1985 Sep 5. PMID: 3930755 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |