| General Information |
| MoonProt ID | 361 |
| First appeared in release | 2.0 |
| Name(s) | homocitrate synthase, Lys20, Homocitrate synthase, cytosolic isozyme
Gene: LYS20 |
| UniProt ID | P48570 (HOSC_YEAST) |
| GO terms | GO:0001207 histone displacement
GO:0006281 DNA repair
GO:0008652 cellular amino acid biosynthetic process
GO:0009085 lysine biosynthetic process
GO:0019752 carboxylic acid metabolic process
GO:0019878 lysine biosynthetic process via aminoadipic acid
GO:0003824 catalytic activity
GO:0004410 homocitrate synthase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0046912 transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
GO:0005634 nucleus
GO:0005737 cytoplasm |
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae (yeast, fungi) |
| Sequence length | 428 amino acids |
| FASTA sequence | >sp|P48570|HOSC_YEAST Homocitrate synthase, cytosolic isozyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LYS20 PE=1 SV=1
MTAAKPNPYAAKPGDYLSNVNNFQLIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDYIELTSPVASEQSRKDCEAICKLGLKAKILTHIRCHMDDAKVAVETGVDGVDVVIGTSKFLRQYSHGKDMNYIAKSAVEVIEFVKSKGIEIRFSSEDSFRSDLVDLLNIYKTVDKIGVNRVGIADTVGCANPRQVYELIRTLKSVVSCDIECHFHNDTGCAIANAYTALEGGARLIDVSVLGIGERNGITPLGGLMARMIVAAPDYVKSKYKLHKIRDIENLVADAVEVNIPFNNPITGFCAFTHKAGIHAKAILANPSTYEILDPHDFGMKRYIHFANRLTGWNAIKARVDQLNLNLTDDQIKEVTAKIKKLGDVRSLNIDDVDSIIKNFHAEVSTPQVLSAKKNKKNDSDVPELATIPAAKRTKPSA |
| Structure Information |
| PDB ID | |
| Quaternary structure | |
| SCOP | |
| CATH | |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-15, 400-428 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | homocitrate synthase, enzyme, lysine biosynthetic pathway |
| References for function | 8923736 |
| E.C. number | 2.3.3.14 |
| Location of functional site(s) | |
| Cellular location of function | |
| Comments | |
| Function 2 |
| Function description | DNA damage repair, helps in INO80 accumulation at DNA breaks to aid in histone eviction |
| References for function | 25628362, 20810648 |
| E.C. number | |
| Location of functional site(s) | C-terminal domain. Torres-Machorro AL, Aris JP, Pillus L. 2015. A moonlighting metabolic protein influences repair at DNA double-stranded breaks. Nucleic Acids Res 43:1646-1658. PMID: 25628362. |
| Cellular location of function | nucleus, chromatin |
| Comments | a mutation in catalytic activity can still function in DNA repair. Scott,E.M. and Pillus,L. (2010) Homocitrate synthase connects amino acid metabolism to chromatin functions through Esa1 and DNA damage. Genes Dev., 24, 1903–1913. PMID: 20810648 |