Protein Information

General Information
MoonProt ID412
First appeared in release3.0
Name(s)RING-type E3 ubiquitin transferase BRCA1; gene: brca1.L
UniProt IDQ90X96
GO termsGO:0004842 ubiquitin-protein transferase activity GO:0006281 DNA repair GO:0008270 zinc ion binding GO:0046872 metal ion binding GO:0003677 DNA binding GO:0005634 nucleus GO:0006974 cellular response to DNA damage stimulus GO:0046872 metal ion binding GO:0005694 chromosome GO:0007049 cell cycle GO:0005515 protein binding GO:0005694 chromosome GO:0005634 nucleus GO:0005737 cytoplasm GO:0016567 protein ubiquitination
Organisms for which functions have been demonstratedXenopus laevis (African clawed frog)
Sequence length1,579 amino acids
FASTA sequence>tr|Q90X96|Q90X96_XENLA RING-type E3 ubiquitin transferase BRCA1 OS=Xenopus laevis OX=8355 GN=brca1.L PE=2 SV=1 MTCSRMDIEGICSVISVMQKNLECPICLELMKEPVATKCDHIFCKFCMLQLLSKKKKGTVPCPLCKTEVTRRSLQESHRFKLLVEGQLKIIKAFEFDSGYKFFPSQEHTKGLDSTIEDVLVKEDQSIVHCKGYRNRKKGVFNRKTYEETGMLSVSKAEEQFAKEVTRLIPCRQKKPKKEAALIFSNCVPDSSDGDLLNKENGLRNDCSPLHYEKEDTQIPEMEEMVESDLAECEFAESAGSNLLGFDGPEGIPEISAETSINAAGNCDFYGRKTEQFPNDHHCSFKQNIADAEQNKRNQHCGNVPFAPMGKSNLDEKETVETDFDNQHNDSNPENNDPLGKVTKLMRRSTERVNEWLLKTNQDFSTLSAEEDPILDALALQNKETSDKRSCSSDDSELMPVLHKHAEKGISGGGFDKPAVGVKDKIFCKVYKRERKAMPPNNITCVAEVHHDSALETGKENMTLEYGTGMSHLMSKRKMVYSLNPENTSKKNDLANINGSINVFPDCISDANLELEDKSEADSNSADACQSDIVHSSNTQIKQQCVESLANAGETRKKQELSCERSQEEKDFTQSGALGPKTRSQKSPYGHSELHIESSQISNEPSNVTKQVEVRRSRRLLMLPKGPGNKSNSNAVKEMNEQENIAQIPEFRVKDTNKNNEISDTVPSQLKKKDTYFPPCTSEDTGELENGIPVRKISDQNASLDNPIDPCKDEYSDTLLHVAGDNVQPDYMETEESELETQHIVKMFKTSKRTSFILESKEAENENRVNSAVEISQVETSNELPNVAECRHTSLLSSAKEQSGALLKQGSPSSEPKKTSPIHMLKKTESKHSKMSRNRRGKVKPSSNSAKNTTGQPDNLNNPTQGVTGSLYNKQVMSDFPMRLNVDEEHTNASAKGSQSSVADKSTAHSNVNTDFISDINSATPDGLLHYMDKAEGNSSPWDTTRDKNAALLESCLPSAYGSTGEKTPIPKVQSSEEGSSQGLFNQKPKCSSSGKADQKNSSKNPGKSQCRILSDFSGSSNNGKCSSQEMLSKAAFPSLSQESQCSVSLFSTQSNMSQQSVDEDHKQDVLQDELVSPNKTKRTAISGTEETQISPLRDQYQNPDIDEASECESEASHTGDSSILSSQDELLNTQQRNYMKDSLKKLQQEMAALEAVLGQHGTQKLEAETTCIPSSEHVTEMQEATEEEEEETYQGENLFVKTPPEHVANGSTLIGQSTDNGLQGRNRPVSPSFLCQTRIEKDTPEIVCPAMGISKKSLL TQKELPSQHKQEIRPDLETVNVTPTNPIDAQESLRQSSKNKRGSRMLHNKRSTSNQGSFCLSVEAAESPQIPKQNKAEFGIARKSTSPTFASPARAKVPSVGFKSPVVSSRRNLSFVASGLNQCEMALVQRFSKTTQSILSSRITDSTTHVIMKTDAELVCERTLKYFQGIASRKWVVSYEWVVQSFREGQILDEYDFEVKGDVINGRNHRGPRRSRLGSDGLLLIDFEICFFGSFTDMTLDDLEWMVSECGATVVKKLQFFKKKHNVTSLVIVQPDASTEVRDYTEIRKKHKALVVTREWLMDSVATYRLQKFDAYLA
Structure Information
PDB IDNA
Quaternary structureNA
SCOPNA
CATHNA
TM Helix Predictionno TM helices
DisProt AnnotationNot in DisProt
Predicted Disorder RegionsUse FASTA sequence on the MFDp2 webserver. moonID_412_uniID_Q90X96 is 1579 residues long, with 886 residues (56.11%) predicted as disordered.The protein has 1 short (< 30 residues) disorder segment and 6 long (>= 30 residues) disorder segments. Segment 1 - Long (>= 30 residues) disordered segment Segment is located between positions 205 and 235 in the sequence. The segment is 31 residues long (1.96 % of the total sequence length). Segment 2 - Long (>= 30 residues) disordered segment Segment is located between positions 281 and 351 in the sequence. The segment is 71 residues long (4.50 % of the total sequence length). Segment 3 - Short (< 30 residues) disordered segment Segment is located between positions 371 and 388 in the sequence. The segment is 18 residues long (1.14 % of the total sequence length). Segment 4 - Long (>= 30 residues) disordered segment Segment is located between positions 515 and 717 in the sequence. The segment is 203 residues long (12.86 % of the total sequence length). Segment 5 - Long (>= 30 residues) disordered segment Segment is located between positions 751 and 915 in the sequence. The segment is 165 residues long (10.45 % of the total sequence length). Segment 6 - Long (>= 30 residues) disordered segment Segment is located between positions 933 and 1139 in the sequence. The segment is 207 residues long (13.11 % of the total sequence length). Segment 7 - Long (>= 30 residues) disordered segment Segment is located between positions 1172 and 1362 in the sequence. The segment is 191 residues long (12.10 % of the total sequence length).
Connections to Disease
OMIM ID
Function 1
Function descriptionphysically interacts with BARD1 and spindle-associated proteins TPX2, NuMa, and XRHAMM, required for spindle formation induced by nuclei in cytoplasmic extracts from Xenopus laevis eggs
References for function17081976
E.C. numberNA
Location of functional site(s)NA
Cellular location of functionspindle poles in cytoplasm
Commentsrequired for spindle formation induced by nuclei in cytoplasmic extracts from Xenopus laevis eggs; immunodepletion of BRCA1 results in abnormal morphology of spindles with unfocused poles and higher MT density compared to non-depleted extracts; studies suggest that BRCA1 directly contribute to centrosome-independent spindle assembly
Function 2
Function descriptionRING-type E3 ubiquitin transferase BRCA1
References for function23747565
E.C. numberNA
Location of functional site(s)NA
Cellular location of functionnucleus
CommentsNA