| General Information |
| MoonProt ID | 6 |
| First appeared in release | 1.0 |
| Name(s) | Phosphoglucose isomerase
PGI
GPI
AMF
NLK
Glucose-6-phosphate isomerase
Autocrine motility factor
Neuroleukin
Differentiation and maturation mediator
Phosphohexose isomerase
PHI |
| UniProt ID | P06745 (G6PI_MOUSE), Reviewed |
| GO terms | GO:0001525 angiogenesis
GO:0001701 in utero embryonic development
GO:0001707 mesoderm formation
GO:0006094 gluconeogenesis
GO:0006096 glycolytic process
GO:0034101 erythrocyte homeostasis
GO:0042593 glucose homeostasis
GO:0004347 glucose-6-phosphate isomerase activity
GO:0005125 cytokine activity
GO:0005515 protein binding
GO:0008083 growth factor activity
GO:0016853 isomerase activity
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0060170 ciliary membrane |
| Organisms for which functions have been demonstrated | Staphylococcus aureus (Gram positive bacterium, causes staph infections, abcesses/boils) |
| Sequence length | 558 |
| FASTA sequence | >sp|P06745|G6PI_MOUSE Glucose-6-phosphate isomerase OS=Mus musculus GN=Gpi PE=1 SV=4
MAALTRNPQFQKLLEWHRANSANLKLRELFEADPERFNNFSLNLNTNHGHILVDYSKNLVNKEVMQMLVELAKSRGVEAARDNMFSGSKINYTENRAVLHVALRNRSNTPIKVDGKDVMPEVNRVLDKMKSFCQRVRSGDWKGYTGKSITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLASLSPETSLFIIASKTFTTQETITNAETAKEWFLEAAKDPSAVAKHFVALSTNTAKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDHFEQLLSGAHWMDQHFLKTPLEKNAPVLLALLGIWYINCYGCETHALLPYDQYMHRFAAYFQQGDMESNGKYITKSGARVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMKGKLPEEARKELQAAGKSPEDLEKLLPHKVFEGNRPTNSIVFTKLTPFILGALIAMYEHKIFVQGIMWDINSFDQWGVELGKQLAKKIEPELEGSSAVTSHDSSTNGLISFIKQQRDTKLE
|
| Structure Information |
| PDB ID | 1U0E 1U0F 1UOG |
| Quaternary structure | Dimer of identical subunits
|
| SCOP | SIS domain |
| CATH | 3.40.50.10490, 1.10.1390.10 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-9,449-457,531-534, 553-558 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | Catalyzes interconversion of glucose-6-phosphate and fructose-6-phosphate in glycolysis and gluconeogenesis |
| References for function | Lohmann, K. (1933) Biochem. Z. 262, 137.
Rose IA, OConnell EL. Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction. J Biol Chem. 1961 Dec;236:3086-92. PMID: 14493830.
Rose IA. Mechanism of the aldose-ketose isomerase reactions. Adv Enzymol Relat Areas Mol Biol. 1975;43:491-517. PMID: 1106126.
Rose IA. Chemistry of proton abstraction by glycolytic enzymes (aldolase, isomerases and pyruvate kinase). Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):131-43. PMID: .6115413
Willem R, Malaisse-Lagae F, Ottinger R, Malaisse WJ. Phosphoglucoisomerase-catalysed interconversion of hexose phosphates. Kinetic study by 13C n.m.r. of the phosphoglucoisomerase reaction in 2H2O. Biochem J. 1990 Jan 15;265(2):519-24. PMID: 2154192.
|
| E.C. number | 5.3.1.9
|
| Location of functional site(s) | Active site pocket containing His388, Glu357, Arg272, Lys518, Thr214, Ser209, Thr211, Ser159
|
| Cellular location of function | Cytoplasm |
| Comments | Multistep catalytic reaction involves ring opening followed by isomerization step, strict specificity for glucose-6-phosphate and fructose-6-phosphate |
| Function 2 |
| Function description | Cytokine/growth factor, binds to target cells and causes pre-B cells to mature into antibody secreting cells, supports survival of embryonal neurons, causes differentiation of some leukemia cell lines |
| References for function | Chaput M, Claes V, Portetelle D, Cludts I, Cravador A, Burny A, Gras H, Tartar A. The neurotrophic factor neuroleukin is 90% homologous with phosphohexose isomerase. Nature 1988 Mar 31;332(6163):454-5. PMID: 3352744.
Faik P, Walker JI, Redmill AA, Morgan MJ. Mouse glucose-6-phosphate isomerase and neuroleukin have identical 3' sequences. Nature. 1988 Mar 31;332(6163):455-7. PMID: 3352745.
Watanabe H, Takehana K, Date M, Shinozaki T, Raz A. Tumor cell autocrine motility factor is the neuroleukin/phosphohexose isomerase polypeptide. Cancer Res. 1996 Jul 1;56(13):2960-3. PMID: 8674049.
Xu W, Seiter K, Feldman E, Ahmed T, Chiao JW. The differentiation and maturation mediator for human myeloid leukemia cells shares homology with neuroleukin or phosphoglucose isomerase. Blood. 1996 Jun 1;87(11):4502-6. PMID: 8639816.
Gurney ME, Heinrich SP, Lee MR, Yin HS. Molecular cloning and expression of neuroleukin, a neurotrophic factor for spinal and sensory neurons. Science. 1986 Oct 31;234(4776):566-74. PMID: 3764429.
Gurney ME, Apatoff BR, Spear GT, Baumel MJ, Antel JP, Bania MB, Reder AT. Neuroleukin: a lymphokine product of lectin-stimulated T cells. Science. 1986 Oct 31;234(4776):574-81. PMID: 3020690.
Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A. Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor. J Biol Chem 1991 Jul 15;266(20):13442-8. PMID: 1649192.
|
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | Extracellular |
| Comments | |