| General Information |
| MoonProt ID | 65 |
| First appeared in release | 1.0 |
| Name(s) | alpha subunit of 6-phosphofructokinase
Phosphofructokinase
Phosphohexokinase
Gene Name:PFK1 |
| UniProt ID | Q92448 (K6PF1_PICPA), Reviewed |
| GO terms | GO:0006002 fructose 6-phosphate metabolic process
GO:0006096 glycolytic process
GO:0008152 metabolic process
GO:0016310 phosphorylation
GO:0046835 carbohydrate phosphorylation
GO:0000166 nucleotide binding
GO:0003824 catalytic activity
GO:0003872 6-phosphofructokinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0005737 cytoplasm
GO:0005945 6-phosphofructokinase complex |
| Organisms for which functions have been demonstrated | Komagataella pastoris (Yeast) (Pichia pastoris) |
| Sequence length | 989 |
| FASTA sequence | >gi|254568386|ref|XP_002491303.1| Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis [Komagataella pastoris GS115]
MPEPSISALSFTSFVTNDDKLFEETFNFYTKLGFHATRSYVKDNRSDFELTGISTDSIKEIWLESFPLSEVVETSAGRELRKPLQESVGYQSEALLGYSPYQSDGVVIKLRLSNHDLQKNKDLPGEVTFFTASIDKLRAKLIEIGAEIIPSEIDLVEFSTKDPMGDVISFSSYPSLSSKKITSPDFFLHPKKEVRSQESIVEQVKSEEGKKKIAIITSGGDAPGMNAAVRAVTRAGIFYGCKVYACYEGYTGLVKGGDMLKELQWQDVRGLLSIGGTIIGTARSKEFRERWGRLQACYNMVSNGIDALVVCGGDGSLTGADLFRNEWPELIKELLGEGKITKEQYETHRNLTIVGLVGSIDNDMCGTDSTIGAYSSLERIIELVDYIDATAASHSRAFVVEVMGRHCGWLGLMSGIATGADYIFIPERPPSETNWKDDLKKVCLRHREKGRRKTTVIVAEGAIDDQLNPITSEEVKDVLVEIGLDTRITRLGHVQRGGAPCAFDRFLATVQGVDAVRAVLESTPAIPSPVISILENKIVRQPLVESVAQTKTVSDAIEAKDFDKALKLRDQEFATSYESFLSVSKYDDGSYLVPESSRLNIAIIHVGAPTSALNPATRVATLNSLAKGHRVFAIRNGFAGLIRHGAVRELNWIDVEDWHNTGGSEIGTNRSLPSDDMGTVAYYFQQYKFDGLIIIGGFEAFTALYQLDAARAQYPIFNIPMCCLPATVSNNVPGTEYSLGSDTCLNTLSGYCDAVKQSASASRRRTFVVEVQGGYSGYLASYAGLITGALAVYTPENPINLQTVQEDIELLTRTYEEDDGKNRSGKIFIHNEKASKVYTTDLIAAIIGEAGKGRFESRTAVPGHVQQGKSPSSIDRVNACRLAIKCCNFIEDANFQVKHNANLSADERHLRFFYDDGVKTSAVSGKSSVIDDNTSVVIGIQGSEVTFTPVKQLWEKETHHKWRKGKNVHWEQLNIVSDLLSGRLSIRTT
|
| Structure Information |
| PDB ID | 3OPY |
| Quaternary structure | |
| SCOP | NA |
| CATH | 3.10.180.90, 3.40.50.450, 3.40.50.460 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | phosphofructokinase, enzyme
ATP + D-fructose 6-phosphate => ADP + D-fructose 1,6-bisphosphate Glycolysis |
| References for function | |
| E.C. number | 2.7.1.11 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | protein, but not catalytic function, is required for microautophagy (vacuolar degradation of peroxisomes)
only alpha subunit of phosphofructokinase plays this role |
| References for function | Yuan W, Tuttle DL, Shi YJ, Ralph GS, Dunn WA. Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase. J Cell Sci. 1997 Aug. PMID: 9296392 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |