| General Information |
| MoonProt ID | 71 |
| First appeared in release | 1.0 |
| Name(s) | Enolase 1
2-phospho-D-glycerate hydro-lyase 1
2-phosphoglycerate dehydratase 1
Gene Name:ENO1 |
| UniProt ID | P00924 (ENO1_YEAST), Reviewed |
| GO terms | GO:0006094 gluconeogenesis
GO:0006096 glycolytic process
GO:0032889 regulation of vacuole fusion, non-autophagic
GO:0000287 magnesium ion binding
GO:0004634 phosphopyruvate hydratase activity
GO:0005515 protein binding
GO:0016829 lyase activity
GO:0046872 metal ion binding
GO:0000015 phosphopyruvate hydratase complex
GO:0000324 fungal-type vacuole
GO:0005737 cytoplasm
GO:0005739 mitochondrion |
| Organisms for which functions have been demonstrated | Saccharomyces cerevisiae (yeast, fungi) |
| Sequence length | 437 |
| FASTA sequence | >sp|P00924|ENO1_YEAST Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
MAVSKVYARSVYDSRGNPTVEVELTTEKGVFRSIVPSGASTGVHEALEMRDGDKSKWMGKGVLHAVKNVNDVIAPAFVKANIDVKDQKAVDDFLISLDGTANKSKLGANAILGVSLAASRAAAAEKNVPLYKHLADLSKSKTSPYVLPVPFLNVLNGGSHAGGALALQEFMIAPTGAKTFAEALRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAEEALDLIVDAIKAAGHDGKIKIGLDCASSEFFKDGKYDLDFKNPNSDKSKWLTGPQLADLYHSLMKRYPIVSIEDPFAEDDWEAWSHFFKTAGIQIVADDLTVTNPKRIATAIEKKAADALLLKVNQIGTLSESIKAAQDSFAAGWGVMVSHRSGETEDTFIADLVVGLRTGQIKTGAPARSERLAKLNQLLRIEEELGDNAVFAGENFHHGDKL
|
| Structure Information |
| PDB ID | 1EBG
2EBH
1ONE
2ONE
2AL1
1P48 and many others |
| Quaternary structure | |
| SCOP | TIM beta/alpha-barrel, Enolase N-terminal domain-like |
| CATH | 3.30.390.10, 3.20.20.120 |
| TM Helix Prediction | no TM helices |
| DisProt Annotation | Not in DisProt |
| Predicted Disorder Regions | 1-4, 431-437 |
| Connections to Disease |
| OMIM ID | |
| Function 1 |
| Function description | enolase, enzyme
2-phospho-D-glycerate <=> phosphoenolpyruvate + H2O
Glycolysis |
| References for function | |
| E.C. number | 4.2.1.11 |
| Location of functional site(s) | |
| Cellular location of function | cytoplasm |
| Comments | |
| Function 2 |
| Function description | required for vacuole homotypic membrane fusion and protein trafficking to the vacuole
enzyme catalytic activity not needed for this role |
| References for function | Decker BL, Wickner WT. Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast. J Biol Chem. 2006 May 19. PMID: 16565073 |
| E.C. number | N/A |
| Location of functional site(s) | |
| Cellular location of function | Vacuole, surface of the vacuole |
| Comments | |